論文 - 詳細
| RRC ID | 87690 |
|---|---|
| 著者 | Chelysheva L, Grandont L, Vrielynck N, le Guin S, Mercier R, Grelon M. |
| タイトル | An easy protocol for studying chromatin and recombination protein dynamics during Arabidopsis thaliana meiosis: immunodetection of cohesins, histones and MLH1. |
| ジャーナル | Cytogenet Genome Res |
| Abstract |
Plant meiosis studies have enjoyed a fantastic boom in recent years with the use of Arabidopsis thaliana as a model not only for molecular genetics and genomics but also for cytogenetics. In this article we describe a new protocol for immunolabelling meiotic proteins that allows the detection of a large range of proteins on strongly spread chromosomes throughout the entire meiotic process. We used this method to immunodetect MLH1, a crucial component of the meiotic recombination machinery, and found that it can be visualised as foci from pachytene to diakinesis, where it co-localises with chiasmata. The mean MLH1 foci number per meiotic cell at diakinesis was 8.4 for WS-4 and 9.95 for Col-0, with the number of foci per bivalent ranging from 1 to 5. We also analysed MLH1 distribution within bivalents and found that they were not restricted to specific chromosomal regions. The analysis of MLH1 foci formation in the Atzip4 mutant, where class I crossover (CO) formation is prevented, revealed that residual chiasmata were not labelled by MLH1, strongly suggesting that MLH1 antibodies only label class I COs in Arabidopsis. It thus appears that the 'obligatory CO' is systematically labeled by MLH1 and is generated through the class I pathway. |
| 巻・号 | 129(1-3) |
| ページ | 143-53 |
| 公開日 | 2010-7-1 |
| DOI | 10.1159/000314096 |
| PII | 000314096 |
| PMID | 20628250 |
| MeSH | Adenosine Triphosphatases / immunology Adenosine Triphosphatases / metabolism Animals Antibodies Arabidopsis / cytology* Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / immunology Arabidopsis Proteins / metabolism* Cell Cycle Proteins / metabolism Chromatin / genetics Chromatin / metabolism Chromosomal Proteins, Non-Histone / metabolism Chromosomes, Plant / genetics Chromosomes, Plant / metabolism Cohesins Crossing Over, Genetic Cytogenetic Analysis / methods* Histones / metabolism Immunohistochemistry / methods* Meiosis / genetics* Meiosis / physiology MutL Protein Homolog 1 Rats Recombination, Genetic |
| IF | 1.114 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda02005 |