論文 - 詳細
| RRC ID | 87693 |
|---|---|
| 著者 | Peters S, Egert A, Stieger B, Keller F. |
| タイトル | Functional identification of Arabidopsis ATSIP2 (At3g57520) as an alkaline α-galactosidase with a substrate specificity for raffinose and an apparent sink-specific expression pattern. |
| ジャーナル | Plant Cell Physiol |
| Abstract |
Arabidopsis ATSIP2 has recently been suggested to be a raffinose synthase gene. However, it has high amino acid identity to functionally characterized alkaline α-galactosidases from Cucumis melo and Zea mays. Using the Sf9 insect cell expression system, we demonstrate that recombinant ATSIP2 is a genuine alkaline α-galactosidase with a distinct substrate specificity for raffinose, and not a raffinose synthase. A β-glucuronidase reporter construct using the ATSIP2 promoter shows that ATSIP2 is strongly expressed in sink tissues of Arabidopsis, i.e. sink leaves and non-xylem parts of the root stele, suggesting a physiological function in raffinose phloem unloading. |
| 巻・号 | 51(10) |
| ページ | 1815-9 |
| 公開日 | 2010-10-1 |
| DOI | 10.1093/pcp/pcq127 |
| PII | pcq127 |
| PMID | 20739305 |
| MeSH | Animals Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cell Line Gene Expression Regulation, Plant Insecta / cytology Phloem / metabolism* Promoter Regions, Genetic RNA, Plant / genetics Raffinose / metabolism* Recombinant Proteins / genetics Recombinant Proteins / metabolism Substrate Specificity alpha-Galactosidase / genetics alpha-Galactosidase / metabolism* |
| IF | 4.062 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda02775 |