論文 - 詳細
| RRC ID | 87742 |
|---|---|
| 著者 | Kuramochi M, Zhu S, Takanashi C, Yang Y, Arai T, Shinkai Y, Doi M, Mio K, Tsuda S, Sasaki YC. |
| タイトル | A mutation to a fish ice-binding protein synthesized in transgenic Caenorhabditis elegans modulates its cold tolerance. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
A cryoprotectant known as ice-binding protein (IBP) is thought to facilitate the cold survival of plants, insects, and fungi. Here, we prepared a genetically modified Caenorhabditis elegans strain to synthesize fish-derived IBPs in its body wall muscles and examined whether the antifreeze activity modification of this IBP by point mutation affects the cold tolerance of this worm. We chose a 65-residue IBP identified from notched-fin eelpout, for which the replacement of the 20th alanine residue (A20) modifies its antifreeze activity. These mutant proteins are denoted A20L, A20G, A20T, A20V, and A20I along with the wild-type (WT) protein. We evaluated the survival rate (%) of the transgenic C. elegans that synthesized each IBP mutant following 24 h of preservation at -5, +2, and +5 °C. Significantly, a dramatic improvement in the survival rate was detected for the worms synthesizing the activity-enhanced mutants (A20T and A20I), especially at +2 °C. In contrast, the rate was not improved by the expression of the defective mutants (A20L, A20G, WT and A20V). The survival rate (%) probably correlates with the antifreeze activity of the IBP. These data suggest that IBP protects the cell membrane by employing its ice-binding mechanism, which ultimately improves the cold tolerance of an IBP-containing animal. |
| 巻・号 | 628 |
| ページ | 98-103 |
| 公開日 | 2022-11-5 |
| DOI | 10.1016/j.bbrc.2022.08.073 |
| PII | S0006-291X(22)01213-X |
| PMID | 36084557 |
| MeSH | Alanine / genetics Animals Antifreeze Proteins* / chemistry Antifreeze Proteins* / genetics Antifreeze Proteins* / metabolism Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Carrier Proteins / metabolism Fish Proteins / genetics Freezing Ice* Mutant Proteins / metabolism Mutation |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | |