| RRC ID |
87772
|
| Author |
Ansai S, Hosokawa H, Maegawa S, Naruse K, Washio Y, Sato K, Kinoshita M.
|
| Title |
Deficiency of Serotonin in Raphe Neurons and Altered Behavioral Responses in Tryptophan Hydroxylase 2-Knockout Medaka (Oryzias latipes).
|
| Journal |
Zebrafish
|
| Abstract |
Serotonin (5-hydroxytryptamine [5-HT]) is a bioactive monoamine that acts as a neurotransmitter in the central and peripheral nervous system of animals. Teleost fish species have serotonergic neurons in the raphe nuclei of the brainstem; however, the role of 5-HT in the raphe neurons in teleost fish remains largely unknown. Here, we established a medaka (Oryzias latipes) strain with targeted disruption of tryptophan hydroxylase 2 (tph2) gene that is involved in the 5-HT synthesis in the raphe nuclei. Immunohistochemistry and mass spectrometry analysis revealed that the homozygous mutants (tph2Δ13/Δ13) lacked the ability to synthesize 5-HT in the raphe neurons. To investigate the effects of 5-HT deficiency in adult behaviors, the mutant fish were subjected to five behavioral paradigms (diving, open-field, light-dark transition, mirror-biting, and two-fish social interaction). The homozygous mutation caused a longer duration of freezing response in all examined paradigms and reduced the number of entries to the top area in the diving test. In addition, the mutants exhibited a decreased number of mirror-biting in the males and an increased contact time in direct social interaction between the females. These results indicate that this tph2-knockout medaka serves as a good model to analyze the effects of 5-HT deficiency in the raphe neurons.
|
| Volume |
14(6)
|
| Pages |
495-507
|
| Published |
2017-12-1
|
| DOI |
10.1089/zeb.2017.1452
|
| PII |
10.1089/zeb.2017.1452
|
| PMID |
28933662
|
| MeSH |
Animals
Animals, Genetically Modified / genetics*
Animals, Genetically Modified / growth & development
Animals, Genetically Modified / metabolism
Behavior, Animal
Female
Male
Neurons / metabolism*
Oryzias / genetics*
Oryzias / growth & development
Oryzias / metabolism
Raphe Nuclei / metabolism*
Serotonin / deficiency*
Tryptophan Hydroxylase / physiology*
|
| IF |
1.845
|
| Resource |
| Medaka |
|
