| RRC ID |
87870
|
| 著者 |
Seki T, Miyanari K, Shiraishi A, Tsuda S, Ansai S, Takeuchi H.
|
| タイトル |
Establishment of a transgenic strain for the whole brain calcium imaging in larval medaka fish (Oryzias latipes).
|
| ジャーナル |
Neurosci Res
|
| Abstract |
GCaMP-based calcium imaging is a powerful tool for investigating neural function in specific neurons. We generated transgenic (Tg) medaka strains expressing jGCaMP7s across extensive brain regions under the control of the gap43 promoter. Using these Tg larvae, calcium imaging successfully detected a tricaine-induced suppression of spontaneous neural activity and topographical visual responses in the optic tectum elicited by moving paramecia or optical fiber stimulation. These results indicate that our Tg medaka strains provide a versatile platform for investigating neural dynamics and their responses to various stimuli.
|
| 巻・号 |
219
|
| ページ |
104944
|
| 公開日 |
2025-10-1
|
| DOI |
10.1016/j.neures.2025.104944
|
| PII |
S0168-0102(25)00127-0
|
| PMID |
40769387
|
| MeSH |
Animals
Animals, Genetically Modified
Brain* / metabolism
Calcium* / metabolism
Larva
Neurons / metabolism
Neurons / physiology
Oryzias* / genetics
Superior Colliculi / physiology
|
| IF |
2.645
|
| リソース情報 |
| メダカ |
hi-medaka (MT835) |
