論文 - 詳細
| RRC ID | 87997 |
|---|---|
| 著者 | Maniates KA, Suryanarayanan S, Rumin A, Lewin M, Singson A, Wehman AM. |
| タイトル | Sperm activation for fertilization requires robust activity of the TAT-5 lipid flippase. |
| ジャーナル | Dev Biol |
| Abstract |
During fertilization, sperm and egg membranes signal and fuse to form a zygote and begin embryonic development. As lipids participate in signaling and membrane fusion, we investigated the role of lipid asymmetry in gametogenesis, fertilization, and embryogenesis. We show that the lipid flippase TAT-5, an essential P4-ATPase that maintains phosphatidylethanolamine asymmetry, is required for both oocyte formation and sperm activation, albeit at different levels of flippase activity. Loss of TAT-5 significantly decreases fertility in both males and hermaphrodites and decreases sperm activation. TAT-5 localizes to the plasma membrane of primary spermatocytes but is sorted away from maturing spermatids after meiosis. We also find that phosphatidylethanolamine asymmetry is lost in residual bodies prior to phosphatidylserine exposure. Our findings demonstrate that phosphatidylethanolamine asymmetry plays key roles during gametogenesis and sperm activation, expanding the roles of lipid dynamics in developmental cell fusion. |
| 巻・号 | 528 |
| ページ | 66-78 |
| 公開日 | 2025-12-1 |
| DOI | 10.1016/j.ydbio.2025.09.003 |
| PII | S0012-1606(25)00252-0 |
| PMID | 40915529 |
| PMC | PMC13045937 |
| MeSH | Adenosine Triphosphatases* / metabolism Animals Cell Membrane / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster Female Fertilization* / physiology Male Meiosis Oocytes / metabolism Phosphatidylethanolamines / metabolism Spermatocytes / metabolism Spermatozoa* / metabolism Spermatozoa* / physiology |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.7 |
| リソース情報 | |
| 線虫 | tm1741 tmIs1200 |