Reference - Detail
|Author||Inoue S, Tahara K, Kaneko T, Ajiki T, Takeda S, Sato Y, Hakamata Y, Murakami T, Takahashi M, Kaneko M, Kobayashi E.|
|Title||Long-lasting donor passenger leukocytes after hepatic and intestinal transplantation in rats.|
BACKGROUND:Donor passenger leukocytes (DPLs) that migrate after organ transplantation stimulate the recipient immune system and normally cause rejection and graft vs. host reaction. However, DPLs also contribute to the unresponsiveness to the donor organ. The quantity and quality of these migrating cells are considered dependent on individual transplanted organs. We compared the DPLs of the liver, which might contain somatic stem cells, with those of intestinal grafts that have highly immunogenetic cells. To study DPLs over a long period, we used green fluorescent protein (GFP) transgenic (Tg) rats developed by us as donors.
METHODS:We performed orthotopic liver transplantation (OLT) and small bowel transplantation (SBT) from GFP Tg rats to wild recipients. A short course of tacrolimus (0.64 mg/kg, intramuscularly) was used to prevent antigenicity of the GFP. The fate of the DPLs in the peripheral blood and the recipient bone marrow was monitored by flow cytometry. Using long-surviving recipients, the GFP(+) cells in the graft and various host immunologic organs were measured and characterized by immunohistochemical staining.
RESULTS:In both groups, the numbers of the GFP(+) cells in the peripheral blood increased transiently and then gradually decreased to undetectable levels. While no GFP(+) cells were identified in the long-surviving-recipient bone marrow, there were a few GFP(+) cells in the graft liver, graft mesenteric lymph nodes and the recipient spleen. These cells showed major histocompatibility complex (MHC) class II antigen. There was no significant difference in the migration patterns of the GFP(+) cells in the OLT and SBT rats.
CONCLUSIONS:In both the OLT and SBT groups, the DPLs migrated transiently in the recipient peripheral blood. A small numbers of MHC class II-positive DPLs were present at the graft site and in the host spleen, but not in the bone marrow. There were no significant differences in the migration patterns of the DPLs between the OLT and SBT rats over the long term.
|MeSH||Animals Animals, Genetically Modified Body Weight Bone Marrow Cells / cytology Bone Marrow Cells / immunology Cell Survival Flow Cytometry Genes, Reporter / genetics Green Fluorescent Proteins Immunohistochemistry Intestine, Small / cytology* Intestine, Small / immunology Intestine, Small / surgery Intestine, Small / transplantation* Leukocytes / cytology* Leukocytes / immunology Leukocytes / metabolism Liver / cytology Liver / immunology Liver / surgery Liver Transplantation* / immunology Luminescent Proteins / genetics Luminescent Proteins / metabolism Lymph Nodes / cytology Lymph Nodes / immunology Male Rats Rats, Wistar Spleen / cytology Spleen / immunology Survival Rate Tacrolimus / pharmacology Tissue Donors*|
|WOS Category||TRANSPLANTATION IMMUNOLOGY|