論文 - 詳細
| RRC ID | 88044 |
|---|---|
| 著者 | Caro L, Wei AD, Thomas CA, Posch G, Uremis A, Franzi MC, Abell SJ, Laszlo AH, Gundlach JH, Ramirez JM, Ailion M. |
| タイトル | An animal toxin-antidote system kills cells by creating a novel cation channel. |
| ジャーナル | PLoS Biol |
| Abstract |
Toxin-antidote systems are selfish genetic elements composed of a linked toxin and antidote. The peel-1 zeel-1 toxin-antidote system in C. elegans consists of a transmembrane toxin protein PEEL-1 which acts cell autonomously to kill cells. Here we investigate the molecular mechanism of PEEL-1 toxicity. We find that PEEL-1 requires a small membrane protein, PMPL-1, for toxicity. Together, PEEL-1 and PMPL-1 are sufficient for toxicity in a heterologous system, HEK293T cells, and cause cell swelling and increased cell permeability to monovalent cations. Using purified proteins, we show that PEEL-1 and PMPL-1 allow ion flux through lipid bilayers and generate currents which resemble ion channel gating. Our work suggests that PEEL-1 kills cells by co-opting PMPL-1 and creating a cation channel. |
| 巻・号 | 23(5) |
| ページ | e3003182 |
| 公開日 | 2025-5-1 |
| DOI | 10.1371/journal.pbio.3003182 |
| PII | PBIOLOGY-D-24-03319 |
| PMID | 40424258 |
| PMC | PMC12136403 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cations / metabolism HEK293 Cells Humans Ion Channel Gating Ion Channels* / metabolism Lipid Bilayers / metabolism Toxins, Biological* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.2 |
| リソース情報 | |
| 線虫 | tm3419 |