| RRC ID |
88044
|
| 著者 |
Caro L, Wei AD, Thomas CA, Posch G, Uremis A, Franzi MC, Abell SJ, Laszlo AH, Gundlach JH, Ramirez JM, Ailion M.
|
| タイトル |
An animal toxin-antidote system kills cells by creating a novel cation channel.
|
| ジャーナル |
PLoS Biol
|
| Abstract |
Toxin-antidote systems are selfish genetic elements composed of a linked toxin and antidote. The peel-1 zeel-1 toxin-antidote system in C. elegans consists of a transmembrane toxin protein PEEL-1 which acts cell autonomously to kill cells. Here we investigate the molecular mechanism of PEEL-1 toxicity. We find that PEEL-1 requires a small membrane protein, PMPL-1, for toxicity. Together, PEEL-1 and PMPL-1 are sufficient for toxicity in a heterologous system, HEK293T cells, and cause cell swelling and increased cell permeability to monovalent cations. Using purified proteins, we show that PEEL-1 and PMPL-1 allow ion flux through lipid bilayers and generate currents which resemble ion channel gating. Our work suggests that PEEL-1 kills cells by co-opting PMPL-1 and creating a cation channel.
|
| 巻・号 |
23(5)
|
| ページ |
e3003182
|
| 公開日 |
2025-5-1
|
| DOI |
10.1371/journal.pbio.3003182
|
| PII |
PBIOLOGY-D-24-03319
|
| PMID |
40424258
|
| PMC |
PMC12136403
|
| MeSH |
Animals
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Cations / metabolism
HEK293 Cells
Humans
Ion Channel Gating
Ion Channels* / metabolism
Lipid Bilayers / metabolism
Toxins, Biological* / metabolism
|
| リソース情報 |
| 線虫 |
tm3419 |
