| RRC ID |
88095
|
| 著者 |
Agostini L, Pfister JA, Basnet N, Ding J, Zhang R, Biertümpfel C, O'Connell KF, Mizuno N.
|
| タイトル |
Structural insights into SSNA1 self-assembly and its microtubule binding for centriole maintenance.
|
| ジャーナル |
Nat Commun
|
| Abstract |
SSNA1 is a fibrillar protein involved in dynamic microtubule remodeling, including nucleation, co-polymerization, and microtubule branching. The underlying molecular mechanism has remained unclear due to a lack of structural information. Here, we determine the cryo-EM structure of C.elegans SSNA-1 at 4.55-Å resolution and evaluate its role in embryonic development. We find that SSNA-1 forms an anti-parallel coiled-coil, with self-assembly facilitated by an overhang of 16 C-terminal residues that form a triple-stranded helical junction. The microtubule-binding region is within the triple-stranded junction, suggesting that self-assembly of SSNA-1 creates hubs for effective microtubule interaction. Genetical analysis elucidates that SSNA-1 deletion significantly reduces embryonic viability, and causes multipolar spindles during cell division. Interestingly, impairing SSNA-1 self-assembly has a comparable effect on embryonic viability as the knockout strain. Our study provides molecular insights into SSNA-1's self-assembly and its role in microtubule binding and cell division regulation through centriole stability.
|
| 巻・号 |
16(1)
|
| ページ |
7512
|
| 公開日 |
2025-8-13
|
| DOI |
10.1038/s41467-025-62696-9
|
| PII |
10.1038/s41467-025-62696-9
|
| PMID |
40804232
|
| PMC |
PMC12350680
|
| MeSH |
Animals
Caenorhabditis elegans* / embryology
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / metabolism
Cell Division
Centrioles* / metabolism
Cryoelectron Microscopy
Embryonic Development
Microtubules* / metabolism
Microtubules* / ultrastructure
Protein Binding
|
| リソース情報 |
| 線虫 |
tm9713
tmIs1245 |
