| RRC ID |
88155
|
| Author |
Noblett N, Roenspies T, Flibotte S, Colavita A.
|
| Title |
Nuclear hormone receptor regulation of PAL-1/Caudal mediates ventral nerve cord assembly in C. elegans.
|
| Journal |
Dev Biol
|
| Abstract |
The regulatory network governed by CDX/Caudal family transcription factors plays critical roles in shaping embryonic neural development. In C. elegans, we found that proper expression of pal-1, the C. elegans Caudal homologue, is required for correct positioning of motor neuron cell bodies in the first larval stage ventral nerve cord (VNC). We identified an upstream regulatory region within the pal-1 promoter that drives pal-1 expression in a subset of DD and DA neuronal progenitors. We also show that SEX-1, a nuclear hormone receptor, is required for motor neuron positioning in the VNC. Loss of sex-1 results in neuronal positioning defects similar to those observed in pal-1 mutants. This is in part due to a requirement for SEX-1 in promoting pal-1 expression in DD and DA neuronal progenitors during VNC assembly. Double mutant analysis further suggests that sex-1 also has pal-1-independent functions. Together, these findings define a transcriptional hierarchy in which the SEX-1 nuclear hormone receptor regulates the tissue-specific activity of PAL-1 to promote proper motor neuron positioning in the VNC and highlight a conserved role for NHR and CDX/Caudal family proteins in central nerve cord formation.
|
| Volume |
528
|
| Pages |
13-26
|
| Published |
2025-12-1
|
| DOI |
10.1016/j.ydbio.2025.08.023
|
| PII |
S0012-1606(25)00240-4
|
| PMID |
40902841
|
| MeSH |
Animals
Caenorhabditis elegans* / embryology
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Gene Expression Regulation, Developmental
Homeodomain Proteins* / genetics
Homeodomain Proteins* / metabolism
Motor Neurons / cytology
Motor Neurons / metabolism
Mutation
Promoter Regions, Genetic
Receptors, Cytoplasmic and Nuclear* / genetics
Receptors, Cytoplasmic and Nuclear* / metabolism
Transcription Factors* / genetics
Transcription Factors* / metabolism
|
| Resource |
| C.elegans |
tm1422 |
