論文 - 詳細
| RRC ID | 88179 |
|---|---|
| 著者 | Rei Yagasaki, Ryo Nakamura, Yuuki Shikaya, Ryosuke Tadokoro, Ruolin Hao, Zhe Wang, Mototsugu Eiraku, Masafumi Inaba, Yoshiko Takahashi |
| タイトル | The gut contractile organoid for studying the gut motility regulated by coordinating signals between interstitial cells of Cajal and smooth muscles |
| ジャーナル | eLife |
| Abstract |
The gut undergoes peristaltic movements regulated by intricate cellular interactions. How these interactions emerge in the developing gut remains poorly explored due to a lack of model system. We here developed a novel contractile organoid that is derived from the muscle layer of chicken embryonic hindgut. The organoid contained smooth muscle cells (SMCs) and interstitial cells of Cajal (ICCs; pacemaker) with few enteric neurons and underwent periodic contractions. The organoid was formed by self-organization with morphological arrangements of ICCs (internal) and SMCs (peripheral), allowing identification of these cells in live. GCaMP-Ca
2+
imaging analyses revealed that Ca
2+
transients between ICC-ICC, SMC-SMC, or SMC-ICC were markedly coordinated. Pharmacological studies further suggested a role of gap junctions in ICC-to-SMC signaling, and also possible mechanical feedback from SMC’s contraction to ICC’s pace-making activities. In addition, two organoids with different rhythms became synchronized when mediated by SMCs, unveiling a novel contribution of SMCs to ICC’s pace-making. The gut contractile organoid developed in this study offers a useful model to understand how the rhythm coordination between/among ICCs and SMCs is regulated and maintained during gut development. |
| 巻・号 | 13 |
| 公開日 | 2025-8-22 |
| DOI | 10.7554/elife.97860.3 |
| PMID | 40843588 |
| PMC | PMC12373395 |
| MeSH | Animals Calcium / metabolism Cell Communication Chick Embryo Chickens Gastrointestinal Motility* / physiology Interstitial Cells of Cajal* / physiology Muscle Contraction* Muscle, Smooth* / physiology Myocytes, Smooth Muscle* / physiology Organoids* / physiology Signal Transduction |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ニワトリ・ウズラ | |