| RRC ID |
88203
|
| 著者 |
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A.
|
| タイトル |
Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
|
| ジャーナル |
Dev Cell
|
| Abstract |
How metabolism is rewired during embryonic development is still largely unknown, as it remains a major technical challenge to resolve metabolic activities or metabolite levels with spatiotemporal resolution. Here, we investigated metabolic changes during development of organogenesis-stage mouse embryos, focusing on the presomitic mesoderm (PSM). We measured glycolytic labeling kinetics from 13C-glucose tracing experiments and detected elevated glycolysis in the posterior, more undifferentiated PSM. We found evidence that the spatial metabolic differences are functionally relevant during PSM development. To enable real-time quantification of a glycolytic metabolite with spatiotemporal resolution, we generated a pyruvate FRET-sensor reporter mouse line. We revealed dynamic changes in cytosolic pyruvate levels as cells transit toward a more anterior PSM state. Combined, our approach identifies a gradient of glycolytic activity across the PSM, and we provide evidence that these spatiotemporal metabolic changes are intrinsically linked to PSM development and differentiation.
|
| 巻・号 |
40(4)
|
| ページ |
331-341.e4
|
| 公開日 |
2017-2-27
|
| DOI |
10.1016/j.devcel.2017.01.015
|
| PII |
S1534-5807(17)30042-4
|
| PMID |
28245920
|
| PMC |
PMC5337618
|
| MeSH |
Animals
Carbon Isotopes
Cell Differentiation / genetics
Computer Systems
Embryo, Mammalian / metabolism
Embryonic Development*
Fluorescence Resonance Energy Transfer
Gene Expression Regulation, Developmental
Genes, Reporter
Glucose / metabolism
Glycolysis*
In Situ Hybridization
Kinetics
Mesoderm / embryology*
Mesoderm / metabolism*
Metabolic Flux Analysis
Metabolomics
Mice
Models, Biological
Organ Specificity / genetics
Phenotype
Pyruvic Acid / metabolism
Somites / embryology
Somites / metabolism
Spatio-Temporal Analysis*
|
| IF |
10.092
|
| リソース情報 |
| 実験動物マウス |
RBRC01862 |
