論文 - 詳細
| RRC ID | 88203 |
|---|---|
| 著者 | Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A. |
| タイトル | Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development. |
| ジャーナル | Dev Cell |
| Abstract |
How metabolism is rewired during embryonic development is still largely unknown, as it remains a major technical challenge to resolve metabolic activities or metabolite levels with spatiotemporal resolution. Here, we investigated metabolic changes during development of organogenesis-stage mouse embryos, focusing on the presomitic mesoderm (PSM). We measured glycolytic labeling kinetics from 13C-glucose tracing experiments and detected elevated glycolysis in the posterior, more undifferentiated PSM. We found evidence that the spatial metabolic differences are functionally relevant during PSM development. To enable real-time quantification of a glycolytic metabolite with spatiotemporal resolution, we generated a pyruvate FRET-sensor reporter mouse line. We revealed dynamic changes in cytosolic pyruvate levels as cells transit toward a more anterior PSM state. Combined, our approach identifies a gradient of glycolytic activity across the PSM, and we provide evidence that these spatiotemporal metabolic changes are intrinsically linked to PSM development and differentiation. |
| 巻・号 | 40(4) |
| ページ | 331-341.e4 |
| 公開日 | 2017-2-27 |
| DOI | 10.1016/j.devcel.2017.01.015 |
| PII | S1534-5807(17)30042-4 |
| PMID | 28245920 |
| PMC | PMC5337618 |
| MeSH | Animals Carbon Isotopes Cell Differentiation / genetics Computer Systems Embryo, Mammalian / metabolism Embryonic Development* Fluorescence Resonance Energy Transfer Gene Expression Regulation, Developmental Genes, Reporter Glucose / metabolism Glycolysis* In Situ Hybridization Kinetics Mesoderm / embryology* Mesoderm / metabolism* Metabolic Flux Analysis Metabolomics Mice Models, Biological Organ Specificity / genetics Phenotype Pyruvic Acid / metabolism Somites / embryology Somites / metabolism Spatio-Temporal Analysis* |
| IF | 10.092 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 28 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01862 |