論文 - 詳細
| RRC ID | 88212 |
|---|---|
| 著者 | Nakamura N, Liao J, Soda Y, Takahashi M, Kodera H, Kobari Y, Hirai Y, Takaori-Kondo A, Okada T. |
| タイトル | Optimizing activation and culture conditions for the production of cord blood-derived CAR-T and natural killer cells. |
| ジャーナル | Br J Haematol |
| Abstract |
Cord blood (CB) is gaining attention as a source of chimeric antigen receptor (CAR) T/NK cells. Many studies have focused on the therapeutic effects, but the methods of activating and culturing T/NK cells without excessive exhaustion during the initial stages of the production of CB-derived CAR-T/NK cells are yet to be established. The activation and culture conditions developed for peripheral blood (PB) CAR-T/NK cells have been used for CB-CAR-T/NK cells, but there are differences in the composition and maturity of PB and CB lymphocytes. Thus, this study aims to optimize activation and culture conditions for the production of CB-CAR-T/NK cells. We compared different doses of various cytokines and found that the balance between activation and exhaustion was best with stimulation with a CD3/CD28 agonist followed by 40 U/mL interleukin (IL)-7+ 40 U/mL IL-15 for PB-T cells; 40 U/mL IL-2+ 40 U/mL IL-7+ 40 U/mL IL-15 for CB-T cells; 40 U/mL IL-2+ 40 U/mL IL-15 for PB-NK cells; and 40 U/mL IL-2+ 200 U/mL IL-15 for CB-NK cells. Using killing assays, we confirmed that these cytokine protocols improved the anti-tumour effects. These results will be useful for the development of CB-CAR-T/NK-cell therapies and suggest the potential of these modalities. |
| 巻・号 | 207(5) |
| ページ | 1863-1873 |
| 公開日 | 2025-11-1 |
| DOI | 10.1111/bjh.70139 |
| PMID | 41030086 |
| PMC | PMC12624202 |
| MeSH | Cell Culture Techniques* / methods Cells, Cultured Cytokines / pharmacology Fetal Blood* / cytology Fetal Blood* / immunology Humans Immunotherapy, Adoptive* / methods Interleukin-15 / pharmacology Killer Cells, Natural* / cytology Killer Cells, Natural* / immunology Killer Cells, Natural* / metabolism Lymphocyte Activation* Receptors, Chimeric Antigen* / immunology Receptors, Chimeric Antigen* / metabolism T-Lymphocytes* / cytology T-Lymphocytes* / immunology |
| IF | 5.518 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 研究用ヒト臍帯血幹細胞 | |