論文 - 詳細
| RRC ID | 88270 |
|---|---|
| 著者 | Clements MP, Byrne E, Camarillo Guerrero LF, Cattin AL, Zakka L, Ashraf A, Burden JJ, Khadayate S, Lloyd AC, Marguerat S, Parrinello S. |
| タイトル | The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. |
| ジャーナル | Neuron |
| Abstract |
Schwann cell dedifferentiation from a myelinating to a progenitor-like cell underlies the remarkable ability of peripheral nerves to regenerate following injury. However, the molecular identity of the differentiated and dedifferentiated states in vivo has been elusive. Here, we profiled Schwann cells acutely purified from intact nerves and from the wound and distal regions of severed nerves. Our analysis reveals novel facets of the dedifferentiation response, including acquisition of mesenchymal traits and a Myc module. Furthermore, wound and distal dedifferentiated Schwann cells constitute different populations, with wound cells displaying increased mesenchymal character induced by localized TGFβ signaling. TGFβ promotes invasion and crosstalks with Eph signaling via N-cadherin to drive collective migration of the Schwann cells across the wound. Consistently, Tgfbr2 deletion in Schwann cells resulted in misdirected and delayed reinnervation. Thus, the wound microenvironment is a key determinant of Schwann cell identity, and it promotes nerve repair through integration of multiple concerted signals. VIDEO ABSTRACT. |
| 巻・号 | 96(1) |
| ページ | 98-114.e7 |
| 公開日 | 2017-9-27 |
| DOI | 10.1016/j.neuron.2017.09.008 |
| PII | S0896-6273(17)30843-7 |
| PMID | 28957681 |
| PMC | PMC5626803 |
| MeSH | Animals Cadherins / physiology Cell Differentiation* Cell Movement / physiology Cells, Cultured Cellular Microenvironment / physiology* Female Male Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / physiology* Mice Mice, Transgenic Nerve Regeneration / physiology* Peripheral Nerve Injuries / pathology Peripheral Nerve Injuries / physiopathology* Primary Cell Culture Rats Rats, Transgenic Receptors, Eph Family / physiology Schwann Cells / cytology* Schwann Cells / physiology* Sciatic Nerve / injuries Sciatic Nerve / physiology Transforming Growth Factor beta / genetics Transforming Growth Factor beta / physiology |
| IF | 14.415 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01459 |