論文 - 詳細
| RRC ID | 88301 |
|---|---|
| 著者 | Willetts L, Felix LC, Jacobsen EA, Puttagunta L, Condjella RM, Zellner KR, Ochkur SI, Kim JD, Luo H, Lee NA, Lee JJ, Moqbel R, Lacy P. |
| タイトル | Vesicle-associated membrane protein 7-mediated eosinophil degranulation promotes allergic airway inflammation in mice. |
| ジャーナル | Commun Biol |
| Abstract |
Eosinophil degranulation is a determining factor in allergy-mediated airway pathology. Receptor-mediated degranulation in eosinophils requires vesicle-associated membrane protein 7 (VAMP-7), a principal component of the SNARE fusion machinery. The specific contribution of eosinophil degranulation to allergen-induced airway responses remains poorly understood. We generated mice with VAMP-7 gene deficiency exclusively in eosinophils (eoCRE/V7) from a cross using eosinophil-specific Cre recombinase-expressing mice crossed with VAMP-7f/f mice. Eosinophils from eoCRE/V7 mice showed deficient degranulation responses in vitro, and responses continued to be decreased following ex vivo intratracheal adoptive transfer of eoCRE/V7 eosinophils into IL-5/hE2/EPX-/- mice. Consistent with diminished degranulation responses, reduced airway hyperresponsiveness was observed in ovalbumin-sensitized and challenged eoCRE/V7 mice following methacholine inhalation. Therefore, VAMP-7 mediates eosinophil degranulation both in vitro and ex vivo, and this event augments airway hyperresponsiveness. |
| 巻・号 | 1 |
| ページ | 83 |
| 公開日 | 2018-1-1 |
| DOI | 10.1038/s42003-018-0081-z |
| PII | 10.1038/s42003-018-0081-z |
| PMID | 30271964 |
| PMC | PMC6123774 |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01926 |