論文 - 詳細
| RRC ID | 88396 |
|---|---|
| 著者 | Yaku K, Okabe K, Gulshan M, Takatsu K, Okamoto H, Nakagawa T. |
| タイトル | Metabolism and biochemical properties of nicotinamide adenine dinucleotide (NAD) analogs, nicotinamide guanine dinucleotide (NGD) and nicotinamide hypoxanthine dinucleotide (NHD). |
| ジャーナル | Sci Rep |
| Abstract |
Nicotinamide adenine dinucleotide (NAD) is an important coenzyme that regulates various metabolic pathways, including glycolysis, β-oxidation, and oxidative phosphorylation. Additionally, NAD serves as a substrate for poly(ADP-ribose) polymerase (PARP), sirtuin, and NAD glycohydrolase, and it regulates DNA repair, gene expression, energy metabolism, and stress responses. Many studies have demonstrated that NAD metabolism is deeply involved in aging and aging-related diseases. Previously, we demonstrated that nicotinamide guanine dinucleotide (NGD) and nicotinamide hypoxanthine dinucleotide (NHD), which are analogs of NAD, are significantly increased in Nmnat3-overexpressing mice. However, there is insufficient knowledge about NGD and NHD in vivo. In the present study, we aimed to investigate the metabolism and biochemical properties of these NAD analogs. We demonstrated that endogenous NGD and NHD were found in various murine tissues, and their synthesis and degradation partially rely on Nmnat3 and CD38. We have also shown that NGD and NHD serve as coenzymes for alcohol dehydrogenase (ADH) in vitro, although their affinity is much lower than that of NAD. On the other hand, NGD and NHD cannot be used as substrates for SIRT1, SIRT3, and PARP1. These results reveal the basic metabolism of NGD and NHD and also highlight their biological function as coenzymes. |
| 巻・号 | 9(1) |
| ページ | 13102 |
| 公開日 | 2019-9-11 |
| DOI | 10.1038/s41598-019-49547-6 |
| PII | 10.1038/s41598-019-49547-6 |
| PMID | 31511627 |
| PMC | PMC6739475 |
| MeSH | Aging / metabolism Animals Guanine Nucleotides / biosynthesis Guanine Nucleotides / metabolism* Guanosine Triphosphate / metabolism Inosine Triphosphate / metabolism Mice NAD / analogs & derivatives* NAD / biosynthesis NAD / metabolism Poly(ADP-ribose) Polymerases / metabolism Sirtuins / metabolism |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01462 |