| RRC ID |
88467
|
| Author |
Biswas KK, Ooura C, Higuchi K, Miyazaki Y, Van Nguyen V, Rahman A, Uchimiya H, Kiyosue T, Koshiba T, Tanaka A, Narumi I, Oono Y.
|
| Title |
Genetic characterization of mutants resistant to the antiauxin p-chlorophenoxyisobutyric acid reveals that AAR3, a gene encoding a DCN1-like protein, regulates responses to the synthetic auxin 2,4-dichlorophenoxyacetic acid in Arabidopsis roots.
|
| Journal |
Plant Physiol
|
| Abstract |
To isolate novel auxin-responsive mutants in Arabidopsis (Arabidopsis thaliana), we screened mutants for root growth resistance to a putative antiauxin, p-chlorophenoxyisobutyric acid (PCIB), which inhibits auxin action by interfering the upstream auxin-signaling events. Eleven PCIB-resistant mutants were obtained. Genetic mapping indicates that the mutations are located in at least five independent loci, including two known auxin-related loci, TRANSPORT INHIBITOR RESPONSE1 and Arabidopsis CULLIN1. antiauxin-resistant mutants (aars) aar3-1, aar4, and aar5 were also resistant to 2,4-dichlorophenoxyacetic acid as shown by a root growth assay. Positional cloning of aar3-1 revealed that the AAR3 gene encodes a protein with a domain of unknown function (DUF298), which has not previously been implicated in auxin signaling. The protein has a putative nuclear localization signal and shares homology with the DEFECTIVE IN CULLIN NEDDYLATION-1 protein through the DUF298 domain. The results also indicate that PCIB can facilitate the identification of factors involved in auxin or auxin-related signaling.
|
| Volume |
145(3)
|
| Pages |
773-85
|
| Published |
2007-11-1
|
| DOI |
10.1104/pp.107.104844
|
| PII |
pp.107.104844
|
| PMID |
17905859
|
| PMC |
PMC2048793
|
| MeSH |
2,4-Dichlorophenoxyacetic Acid / pharmacology*
Amino Acid Sequence
Arabidopsis / drug effects*
Arabidopsis / genetics*
Arabidopsis Proteins / genetics*
Cell Cycle Proteins
Chromosome Mapping
Chromosomes, Plant
Clofibric Acid / pharmacology*
Cloning, Molecular
Cullin Proteins
F-Box Proteins
Herbicide Resistance
Herbicides / pharmacology
Molecular Sequence Data
Mutation
Plant Roots / drug effects*
Plant Roots / genetics
Plant Roots / growth & development
Receptors, Cell Surface
|
| IF |
6.902
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
rpc00008 |
