論文 - 詳細
| RRC ID | 88518 |
|---|---|
| 著者 | Nirwane A, Yao Y. |
| タイトル | SMAlow/undetectable pericytes differentiate into microglia- and macrophage-like cells in ischemic brain. |
| ジャーナル | Cell Mol Life Sci |
| Abstract |
Pericytes are multipotent perivascular cells that play important roles in CNS injury. However, controversial findings exist on how pericytes change and whether they differentiated into microglia-like cells after ischemic stroke. This discrepancy is mainly due to the lack of pericyte-specific markers: the "pericyte" population identified in previous studies contained vascular smooth muscle cells (vSMCs) and/or fibroblasts. Therefore, it remains unclear which cell type differentiates into microglia-like cells after stroke. In this study, lineage-tracing technique was used to mark α-smooth muscle actin (SMA)low/undetectable pericytes, vSMCs, and fibroblasts, and their fates were analyzed after ischemic stroke. We found that SMAlow/undetectable pericytes and fibroblasts but not vSMCs substantially proliferated at the subacute phase after injury, and that SMAlow/undetectable pericyte but not vSMCs or fibroblasts differentiated into Iba1+ cells after ischemic stroke. Further imaging flow cytometry analysis revealed that SMAlow/undetectable pericytes differentiated into both microglia and macrophages at day 7 after stroke. These results demonstrate that SMAlow/undetectable pericytes rather than vSMCs or fibroblasts differentiate into both microglia-like and macrophage-like cells after stroke, suggesting that these pericytes may be targeted in the treatment of ischemic stroke. |
| 巻・号 | 79(5) |
| ページ | 264 |
| 公開日 | 2022-4-28 |
| DOI | 10.1007/s00018-022-04322-1 |
| PII | 10.1007/s00018-022-04322-1 |
| PMID | 35482211 |
| PMC | PMC11073453 |
| MeSH | Brain / metabolism Humans Ischemic Stroke* Macrophages Microglia / metabolism Pericytes Stroke* / metabolism |
| IF | 6.496 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC05603 |