| Abstract |
A Gram-negative, white-pigment, rod-shaped bacterial strain YIM B02566T was isolated from the root of Paris polyphylla var. yunnanensis in China. Strain YIM B02566T exhibited optimal growth at 28 °C and pH 7.0 on Reasoner's 2A agar without NaCl supplementation. Phylogenetic analyses based on the 16S rRNA gene sequences and the concatenation of 433 single-copy orthologous genes revealed that strain YIM B02566T formed a distinct lineage in the genus Taklimakanibacter. The sequence similarity of the 16S rRNA gene between YIM B02566T and two known species of genus Taklimakanibacter were 99.12% (T. deserti SYSU D60010T) and 99.05% (T. lacteus SYSU D60012T), respectively. The principal fatty acids were C19:0 cyclo ω8c, C16:0, and summed feature 8. Strain YIM B02566T contained UQ-10 as the sole respiratory quinone. The polar lipids of the strain comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipid, two unidentified aminolipids, and an unidentified polar lipid. The whole-genome analysis indicated that the genome size was 7.2 Mbp, and the genomic DNA G + C content was 62.9%. The digital DNA-DNA hybridization values between strain YIM B02566T and two Taklimakanibacter species were 24.50% (T. deserti SYSU D60010T) and 25.40% (T. lacteus SYSU D60012T). Meanwhile, the ANI and AAI values between strain YIM B02566T and its closest neighbours are well below the proposed thresholds for species determination. Therefore, based on polyphasic analysis, the isolate represents a novel species, for which the name Taklimakanibacter albus is proposed. The type strain is YIM B02566T (= CGMCC 1.18656T = JCM 34643T).
|
