| Abstract |
A Gram-stain-variable, designated as strain 3BR4T, was isolated from a bacterial community capable of efficiently degrading pymetrozine. This community was enriched from the soil of a long-term continuous cropping cotton field in Xinjiang. Strain 3BR4T exhibited growth capabilities at temperatures ranging from 16 to 45 °C (optimum 37 °C), with a pH 5.0-9.0 (optimum pH 7.0), and in the presence of 0-8% (w/v) NaCl (optimum 2.0%). The complete genome of the novel strain was determined to be 7 330 740 bp in size, with a DNA G + C content of 43.5%. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, revealed that strain 3BR4T is affiliated with members of the genus Ammoniphilus. It showed the highest 16S rRNA gene sequence similarity to Ammoniphilus resinae CC-RT-ET (98.3%), followed by Ammoniphilus oxalaticus RAOx-1T (97.1%) and Ammoniphilus oxalivorans RAOx-FST (96.9%). Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain 3BR4T and A. CC-RT-ET, as well as A. RAOx-1T, were calculated to be 70.1%, 68.8% and 13.0%, 12.8%, respectively. Chemotaxonomic analysis indicated that the major respiratory quinone was menaquinone-7 (MK-7), while the major fatty acids were feature 3 (C16:1ω7c and/or C16:1 ω6c), C16:0, iso-C15:0 and C17:0cyclo. The polar lipids identified included diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Based on these phenotypic, phylogenetic, and chemotaxonomic characteristics, strain 3BR4T (= GDMCC 1.4438T = JCM 37210T) is proposed to represents a novel species of the genus Ammoniphilus, for which the name Ammoniphilus soli sp. nov. is proposed.
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