| RRC ID |
88874
|
| 著者 |
Popowitch EB, Boiditswe SC, Patel MZ, Aquino JN, Hurst JH, Steenhoff AP, Cunningham AL, Yoon JW, Seed PC, Kelly MS.
|
| タイトル |
Corynebacterium rhinophilum sp. nov. isolated from the human upper respiratory tract.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Twelve strains of a novel Corynebacterium species were cultured from human respiratory samples collected in the USA and Botswana. Five strains underwent detailed phenotypic and chemotaxonomic characterization, while all 12 strains were included in phylogenomic analyses. Optimal growth was observed on brain heart infusion solid medium and in liquid media, including brain heart infusion and tryptic soy broth when supplemented with Tween 80, a source of the fatty acid oleic acid. Cells were Gram-positive, non-spore-forming, non-motile bacilli that exhibited catalase activity but lacked oxidase activity. Major fatty acids were 18 : 1 ω9c (oleic acid) and 16 : 0 (palmitic acid). Analysis of 16S ribosomal RNA gene sequences identified these strains as belonging to the genus Corynebacterium (family Corynebacteriaceae), with the closest relatives being Corynebacterium accolens and Corynebacterium macginleyi. Whole-genome sequencing revealed that the genomes are 2.47-2.62 Mbp in size and have a G+C content of 58.7-59.0 mol%. Average nucleotide identity and in silico DNA-DNA hybridization values for comparisons of these genomes to those of closely related phylogenetic neighbours were 93.19-93.62% and 19.9-36.3%, respectively. These results indicate that these strains represent a novel species of Corynebacterium, for which we propose the name Corynebacterium rhinophilum sp. nov., with the type strain MSK072T (=DSM 118652T=JCM 37534T).
|
| 巻・号 |
75(7)
|
| 公開日 |
2025-7-1
|
| DOI |
10.1099/ijsem.0.006835
|
| PMID |
40643564
|
| PMC |
PMC12571295
|
| MeSH |
Bacterial Typing Techniques
Base Composition
Corynebacterium* / classification
Corynebacterium* / genetics
Corynebacterium* / isolation & purification
DNA, Bacterial / genetics
Fatty Acids / analysis
Genome, Bacterial
Humans
Nucleic Acid Hybridization
Phylogeny*
RNA, Ribosomal, 16S / genetics
Respiratory System* / microbiology
Sequence Analysis, DNA
United States
Whole Genome Sequencing
|
| リソース情報 |
| 一般微生物 |
JCM37534 |
