論文 - 詳細
| RRC ID | 88877 |
|---|---|
| 著者 | Wu K, Zhou L, Zheng F, Liu L, Yang M, Li J, Sousa DZ, Cheng L. |
| タイトル | Methanosuratincola petrocarbonis gen. nov., sp. nov., a methyl-reducing methanogen isolated from Shengli oil field, and proposal of Methanosuratincolaceae fam. nov., Methanosuratincolales ord. nov. and Methanosuratincolia classis nov. in the phylum Thermoproteota. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
An anaerobic, thermophilic methanogen, designated strain LWZ-6T, was isolated from the Shengli oil field, China. The cells of strain LWZ-6T were non-motile cocci, with a diameter of 0.5-1.0 µm, and formed aggregates. They reduced methanol and monomethylamine into methane, using H2 as an electron donor. Dimethylamine, trimethylamine and methanethiol, H2/CO2, formate, acetate, pyruvate, lactate and glucose were not used as energy sources. Strain LWZ-6T required yeast extract, acetate or CO2 as carbon sources. Strain LWZ-6T grew at 35-65 °C (optimum 55 °C), pH 5.0-8.0 (optimum 6.0-6.5) and 0-60 g l-1 NaCl (optimum 9 g l-1). The genome was 1.54 Mbp with a G+C content of 54.42 mol%. Strain LWZ-6T shared 83.54% 16S rRNA gene sequence identity with Infirmifilum lucidum strain 3507LTT in the class Thermoprotei. Phylogenetic analysis based on the 16S rRNA gene, along with phylogenomic analysis, indicated that strain LWZ-6T belonged to the candidate class 'Candidatus Methanomethylicia', which lacks cultivated representatives. Based on these findings, a new species within a new genus, Methanosuratincola petrocarbonis gen. nov., sp. nov., is proposed for LWZ-6T (=CCAM 1872T=JCM 39528T). In addition, we propose the Methanosuratincolia class. nov. for candidate class 'Ca. Methanomethylicia' represented by Methanosuratincolaceae fam. nov. and Methanosuratincolales ord. nov. within the phylum Thermoproteota. |
| 巻・号 | 75(7) |
| 公開日 | 2025-7-1 |
| DOI | 10.1099/ijsem.0.006839 |
| PMID | 40637718 |
| PMC | PMC12244365 |
| MeSH | Bacterial Typing Techniques Base Composition China Fatty Acids Methane* / metabolism Methanol / metabolism Methylamines / metabolism Oil and Gas Fields* / microbiology Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 19 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 一般微生物 | JCM39528 |