| RRC ID |
88912
|
| Author |
Juszczyk A, Kowalczyk A, Rogala M, Gurgul A, Jasielczuk I, Szmatoła T, Bojko M, Płaza G, Jałowiecki Ł, Borgulat J, Noga T, Wójcik K, Waś K, Duman M, Szmuc J, Głuchowska E, Kosecka-Strojek M, Latowski D.
|
| Title |
Pseudomonas tussilaginis sp. nov., isolated from leaves of coltsfoot growing in mercury-contaminated soil.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Three mercury-resistant isolates of a novel Pseudomonas species were obtained from the leaves of coltsfoot (Tussilago farfara L.) growing in mercury-contaminated soil in Southern Poland. Using the 16S rRNA gene sequence, they were identified as likely Pseudomonas arcuscaelestis isolates. Whole-genome analyses based on phylogenomic analyses and overall genome relatedness indices showed that they are three nearly identical isolates of a novel species, with Pseudomonas alkylphenolica KL28T as the closest type species by overall genome relatedness indices [86.64% average nucleotide identity (ANI), 34.2% digital DNA-DNA hybridization (dDDH)] and with P. arcuscaelestis P66T (83.85% ANI, 28.5% dDDH) and Pseudomonas auratipiscis 119PT (83.66% average nucleotide identity, 28.4% dDDH) as the two closest by maximum likelihood phylogenomic analysis. Moreover, five genomes publicly available in the databases were found. One of the three isolates, Hg5Tf, was further characterized as Gram-negative, motile, facultatively aerobic rods, which can be distinguished from closely related species by the (delayed) positive urease reaction in the API20NE test and by weak growth on pectin, d-lactic acid methyl ester and acetoacetic acid in the BIOLOG Gen III test. Matrix-assisted laser-desorption/ionization time-of-flight profile of Hg5TfT is also distinct from those of closely related species. A novel species of genus Pseudomonas is proposed with the name Pseudomonas tussilaginis sp. nov. and Hg5TfT (=CCM 9461T=PCM 3563T) as its type strain.
|
| Volume |
75(11)
|
| Published |
2025-11-1
|
| DOI |
10.1099/ijsem.0.006968
|
| PMID |
41236807
|
| MeSH |
Bacterial Typing Techniques
DNA, Bacterial / genetics
Genome, Bacterial
Mercury*
Nucleic Acid Hybridization
Phylogeny*
Plant Leaves* / microbiology
Poland
Pseudomonas* / classification
Pseudomonas* / genetics
Pseudomonas* / isolation & purification
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil Microbiology*
Soil Pollutants*
|
| Resource |
| General Microbes |
JCM21532 |
