論文 - 詳細
| RRC ID | 88980 |
|---|---|
| 著者 | Kusumoto A, Matsuzawa K, Itoh M. |
| タイトル | Development of primers for loop-mediated isothermal amplification for Mycoplasma canadense detection. |
| ジャーナル | J Vet Med Sci |
| Abstract |
Bovine mycoplasma mastitis is highly transmittable and hard to treat by chemotherapy. It causes severe economic loss and is considered a major problem for milk production. Mycoplasma canadense is one of the causative agents of mycoplasma mastitis. A primer set to detect M. canadense was developed based on single nucleotide polymorphism-specific loop-mediated isothermal amplification. Using this primer set, 10 fg M. canadense DNA corresponding to the DNA amount of ~13 cells was detected within 40 min. Cross-reactivity with other bovine Mycoplasma spp., Acholeplasma laidlawii, and mastitis-related bacteria was not observed when ≤1 pg DNA was applied. These results would provide a basis for validating future experiments with spiked-milk and field samples for the development of rapid detection of M. canadense. |
| 巻・号 | 87(6) |
| ページ | 575-579 |
| 公開日 | 2025-6-1 |
| DOI | 10.1292/jvms.24-0303 |
| PMID | 40204470 |
| PMC | PMC12159182 |
| MeSH | Animals Cattle DNA Primers* / genetics DNA, Bacterial / genetics Female Mastitis, Bovine* / diagnosis Mastitis, Bovine* / microbiology Milk / microbiology Molecular Diagnostic Techniques Mycoplasma* / genetics Mycoplasma* / isolation & purification Mycoplasma Infections* / diagnosis Mycoplasma Infections* / microbiology Mycoplasma Infections* / veterinary Nucleic Acid Amplification Techniques* / methods Nucleic Acid Amplification Techniques* / veterinary Polymorphism, Single Nucleotide |
| リソース情報 | |
| 一般微生物 | JCM5673 JCM5709 |