論文 - 詳細
| RRC ID | 89041 |
|---|---|
| 著者 | Yamaura K, Takemata N, Kariya M, Osaka A, Ishino S, Yamauchi M, Tamura T, Hamachi I, Takada S, Ishino Y, Atomi H. |
| タイトル | Chromosomal domain formation by archaeal SMC, a roadblock protein, and DNA structure. |
| ジャーナル | Nat Commun |
| Abstract |
In eukaryotes, structural maintenance of chromosomes (SMC) complexes form topologically associating domains (TADs) by extruding DNA loops and being stalled by roadblock proteins. It remains unclear whether a similar mechanism of domain formation exists in prokaryotes. Using high-resolution chromosome conformation capture sequencing, we show that an archaeal homolog of the bacterial Smc-ScpAB complex organizes the genome of Thermococcus kodakarensis into TAD-like domains. We find that TrmBL2, a nucleoid-associated protein that forms a stiff nucleoprotein filament, stalls the T. kodakarensis SMC complex and establishes a boundary at the site-specific recombination site dif. TrmBL2 stalls the SMC complex at tens of additional non-boundary loci with lower efficiency. Intriguingly, the stalling efficiency is correlated with structural properties of underlying DNA sequences. Our study illuminates a eukaryotic-like mechanism of domain formation in archaea and a role of intrinsic DNA structure in large-scale genome organization. |
| 巻・号 | 16(1) |
| ページ | 1312 |
| 公開日 | 2025-2-19 |
| DOI | 10.1038/s41467-025-56197-y |
| PII | 10.1038/s41467-025-56197-y |
| PMID | 39971902 |
| PMC | PMC11840125 |
| MeSH | Archaeal Proteins* / genetics Archaeal Proteins* / metabolism Chromosomes, Archaeal* / genetics Chromosomes, Archaeal* / metabolism DNA, Archaeal* / chemistry DNA, Archaeal* / genetics DNA, Archaeal* / metabolism Genome, Archaeal Nucleic Acid Conformation Thermococcus* / genetics Thermococcus* / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 53 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM9062 |