| RRC ID |
89044
|
| 著者 |
Arzamasov AA, Rodionov DA, Hibberd MC, Guruge JL, Kent JE, Kazanov MD, Leyn SA, Elane ML, Sejane K, Furst A, Bode L, Barratt MJ, Gordon JI, Osterman AL.
|
| タイトル |
Integrative genomic reconstruction reveals heterogeneity in carbohydrate utilization across human gut bifidobacteria.
|
| ジャーナル |
Nat Microbiol
|
| Abstract |
Bifidobacteria are beneficial saccharolytic microbes that are widely used as probiotics or in synbiotic formulations, yet individual responses to supplementation can vary with strain type, microbiota composition, diet and lifestyle, underscoring the need for strain-level insights into glycan metabolism. Here we reconstructed 68 pathways for the utilization of mono-, di-, oligo- and polysaccharides by analysing the distribution of 589 curated metabolic gene functions (catabolic enzymes, transporters and transcriptional regulators) across 3,083 non-redundant Bifidobacterium genomes of human origin. Thirty-eight predicted phenotypes were validated in vitro for 30 geographically diverse strains, supporting genomics-based predictions. Our analysis uncovered extensive inter- and intraspecies functional heterogeneity, including a distinct clade within Bifidobacterium longum that metabolizes α-glucans and Bangladeshi isolates carrying unique gene clusters for xyloglucan and human milk oligosaccharide utilization. This large-scale genomic compendium advances our understanding of bifidobacterial carbohydrate metabolism and can inform the rational design of probiotic and synbiotic formulations tailored to strain-specific nutrient preferences.
|
| 巻・号 |
10(8)
|
| ページ |
2031-2047
|
| 公開日 |
2025-8-1
|
| DOI |
10.1038/s41564-025-02056-x
|
| PII |
10.1038/s41564-025-02056-x
|
| PMID |
40670725
|
| PMC |
PMC12313528
|
| MeSH |
Bifidobacterium* / classification
Bifidobacterium* / genetics
Bifidobacterium* / metabolism
Carbohydrate Metabolism* / genetics
Gastrointestinal Microbiome* / genetics
Genome, Bacterial*
Genomics
Glucans / metabolism
Humans
Metabolic Networks and Pathways / genetics
Milk, Human
Multigene Family
Oligosaccharides / metabolism
Phylogeny
Polysaccharides / metabolism
Probiotics
Xylans / metabolism
|
| リソース情報 |
| 一般微生物 |
JCM12489 |
