論文 - 詳細
| RRC ID | 89067 |
|---|---|
| 著者 | Kumagai A, Mayanagi K, Hayashi S, Nakano S, Ito S, Fujinami D. |
| タイトル | In Vitro Amyloid Formation by a Bacteriocin From Bifidobacterium longum subsp. infantis. |
| ジャーナル | Proteins |
| Abstract |
Bifidobacterium longum subsp. infantis is a probiotic bacterium isolated from human milk-fed infants. This species secretes various metabolites that contribute to gut microbiome development and immune system maturation. In this study, we investigated bacteriocins, ribosomally synthesized peptides that typically exhibit antimicrobial activity. We produced Blon_0434, a B. infantis -derived bacteriocin belonging to the Lactococcin 972 family, by expressing it heterologously in Escherichia coli . Our results demonstrate that recombinant Blon_0434 is secreted via the Sec-dependent pathway but exhibited no detectable antimicrobial activity under the tested conditions. NMR structural analysis suggests that Blon_0434 is thermodynamically unstable, which may account for its inactivity. Unexpectedly, Blon_0434 formed amyloid-like fibrils in vitro, as demonstrated by thioflavin T fluorescence and transmission electron microscopy. The biological implications of Blon_0434 amyloid formation warrant further investigation, particularly regarding microbial interactions and host immune responses. |
| 巻・号 | 93(11) |
| ページ | 1953-1961 |
| 公開日 | 2025-11-1 |
| DOI | 10.1002/prot.70002 |
| PMID | 40525494 |
| MeSH | Amyloid* / chemistry Amyloid* / metabolism Bacteriocins* / chemistry Bacteriocins* / genetics Bacteriocins* / metabolism Bifidobacterium longum subspecies infantis* / chemistry Bifidobacterium longum subspecies infantis* / genetics Bifidobacterium longum subspecies infantis* / metabolism Escherichia coli / genetics Escherichia coli / metabolism Humans Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Peer-review(Publons) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM1222 JCM20135 JCM20131 JCM1233 JCM1157 |