Reference - Detail
| RRC ID | 89087 |
|---|---|
| Author | Kivenson V, Peters SL, Borrel G, Kivenson A, Roe LT, Hamlish NX, Fadhlaoui K, Schepartz A, Gribaldo S, Hettich RL, Banfield JF. |
| Title | An archaeal genetic code with all TAG codons as pyrrolysine. |
| Journal | Science |
| Abstract |
Multiple genetic codes developed during the evolution of eukaryotes and bacteria, yet no alternative genetic code is known for archaea. We used proteomics to confirm our prediction that certain archaea consistently incorporate pyrrolysine (Pyl) at TAG codons, supporting an alternative archaeal genetic code that we designate the Pyl code. This genetic code has 62 sense codons encoding 21 amino acids. In contrast to monophyletic genetic code distributions in bacteria, the archaeal Pyl code occurs sporadically, indicating that it arose independently in multiple lineages. We discovered that more than 1800 archaeal proteins contain Pyl, increasing the number of such proteins by two orders of magnitude. Additionally, five Pyl transfer RNA (tRNA) pyrrolysyl-tRNA synthetase pairs from Pyl-code archaea were used to introduce Pyl analogs into proteins in Escherichia coli. |
| Volume | 390(6775) |
| Pages | eadu2404 |
| Published | 2025-11-20 |
| DOI | 10.1126/science.adu2404 |
| PMID | 41264687 |
| MeSH | Amino Acyl-tRNA Synthetases / genetics Amino Acyl-tRNA Synthetases / metabolism Archaeal Proteins* / chemistry Archaeal Proteins* / genetics Archaeal Proteins* / metabolism Codon, Terminator* / genetics Escherichia coli / genetics Evolution, Molecular Genetic Code* Lysine* / analogs & derivatives Lysine* / chemistry Lysine* / genetics Methanosarcina* / genetics |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Bluesky |
| Total number of mentions | 225 |
| Altmetric score changes over past 6months | 6.3 |
| Resource | |
| General Microbes | JCM31474 |