| 著者 |
Liu Y, Liu L, Wang Z, Huang X, Dong Y, Hu D, Gu C, Wang ET, Wang H.
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| Abstract |
Rhizobial isolates from Phaseolus vulgaris (common bean) grown in Heilongjiang Province were grouped into two clusters based upon the phylogenies of 16S rRNA and recA-atpD genes representing by BJ04T, MJ22, MJ37, BC56, MC62 and MC63T, MJ21, MJ31, respectively. The phylogenetic analysis of whole genomes further revealed that BJ04T, MJ22, MJ37, BC56 and MC62 formed a cluster neighbored Rhizobium chutanense C5T, while MC63T, MJ21 and MJ31 formed a cluster most related with Rhizobium croatiense 13TT. The whole genome average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the strains BJ04T and R. chutanense C5T and between MC63T and R. croatiense 13TT were lower than the species thresholds 95 % and 70 %, respectively. While the G + C content of the whole genome of the novel strains were 61.16-61.56 %, within the allowable GC content range of the Rhizobium genus (57-66 %). These results, combined with chemical classification and phenotype analysis, supported that BJ04T and MC63T represented two novel species and the names Rhizobium binxianense sp. nov. and Rhizobium mulingense sp. nov. were described for them. BJ04T (=CCTCC AB 2022367T, = JCM 35885T) and MC63T (=CCTCC AB 2024025T, = JCM 36652T) were designed as the type strains, respectively for these two novel species.
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