| RRC ID |
89124
|
| 著者 |
Hikage R, Tadika Y, Asanuma H, Han Y, Nishiyama KI.
|
| タイトル |
MucA is a small peptide encoded by an overlapping sequence with cdsA that upregulates the biosynthesis of glycolipid MPIase in the cold.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
MPIase is a glycolipid involved in protein insertion into and preprotein translocation across the cytoplasmic membranes of E. coli. MPIase is upregulated in the cold conditions to overcome the cold-sensitive protein export. CdsA, a CDP-diacylglycerol synthase, catalyzes the first reaction in MPIase biosynthesis. An open reading frame for a peptide of 50 amino acids is encoded immediately after ispU, a neighboring upstream gene of cdsA, and overlaps cdsA to a large extent. Mutational analysis revealed that the expression of this peptide is essential for upregulation of MPIase in the cold. Consistently, expression of this peptide in trans resulted in cold upregulation of MPIase. We therefore named this peptide MucA after its function (MPIase upregulation in the cold). When the partially purified MucA was added to the reaction of the intermediate in MPIase biosynthesis, a significant increase in the product formation was observed, supporting the function of MucA. The possible role of MucA in MPIase biosynthesis is discussed.
|
| 巻・号 |
721
|
| ページ |
150148
|
| 公開日 |
2024-8-20
|
| DOI |
10.1016/j.bbrc.2024.150148
|
| PII |
S0006-291X(24)00684-3
|
| PMID |
38781662
|
| MeSH |
Amino Acid Sequence
Cold Temperature*
Escherichia coli* / genetics
Escherichia coli* / metabolism
Escherichia coli Proteins* / genetics
Escherichia coli Proteins* / metabolism
Gene Expression Regulation, Bacterial
Glycolipids / biosynthesis
Glycolipids / metabolism
Membrane Transport Proteins
Nucleotidyltransferases
Peptides / chemistry
Peptides / genetics
Peptides / metabolism
Up-Regulation
|
| リソース情報 |
| 原核生物(大腸菌) |
ME9783 |
