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RRC ID 89131
Author Akiyama K, Yano K, Niki H.
Title Involvement of the inner surface residues of bacterial SMC protein MukB in the ssDNA binding in vitro.
Journal Commun Biol
Abstract The bacterial condensin MukB facilitates proper chromosome segregation in Escherichia coli. MukB protein localizes at the ori adjacent region by unknown mechanism. The MukB protein entraps the single-stranded DNA (ssDNA) molecule more efficiently than double-stranded DNA (dsDNA). In the bacterial genome, several copies of the rrn genes are encoded near the ori region. The rrn regions are expected to efficiently generate ssDNA due to their high transcriptional activity and the frequent formation of R-loops. In this study, we identified residues involved in DNA binding. The mutations impaired ssDNA binding more severely than dsDNA binding in vitro, and also caused deficiencies in cell growth and nucleoid segregation. These amino acid residues are aligned and are thought to bind DNA when the MukB dimer entraps a DNA molecule within its ring, thereby likely enhancing the DNA-binding activity of MukB. These residues may contribute to the accumulation of MukB on the chromosome, including the rrn regions.
Volume 9(1)
Pages 79
Published 2025-12-16
DOI 10.1038/s42003-025-09345-5
PII 10.1038/s42003-025-09345-5
PMID 41402526
PMC PMC12820191
MeSH Bacterial Proteins / metabolism Chromosomal Proteins, Non-Histone* / chemistry Chromosomal Proteins, Non-Histone* / genetics Chromosomal Proteins, Non-Histone* / metabolism DNA, Bacterial / genetics DNA, Bacterial / metabolism DNA, Single-Stranded* / genetics DNA, Single-Stranded* / metabolism DNA-Binding Proteins* / chemistry DNA-Binding Proteins* / genetics DNA-Binding Proteins* / metabolism Escherichia coli* / genetics Escherichia coli* / metabolism Escherichia coli Proteins* / chemistry Escherichia coli Proteins* / genetics Escherichia coli Proteins* / metabolism Mutation Protein Binding
Resource
Prokaryotes E. coli