論文 - 詳細
| RRC ID | 89231 |
|---|---|
| 著者 | Jamous M, Hosogane M, Huang X, Suzuki M, Hatano A, Shichino Y, Iwasaki S, Murayama K, Matsumoto M, Nakayama K. |
| タイトル | The ER anchoring and abundance of the EEF1B complex is affected by tissue-specific alternative EEF1D splicing. |
| ジャーナル | Life Sci Alliance |
| Abstract |
The EEF1B complex plays a central role in translation elongation by reactivating EEF1A for the delivery of aminoacyl-tRNAs to the ribosome. Among its components, EEF1D undergoes alternative splicing to produce one long and several short isoforms, each with distinct N-terminal domains and tissue-specific expression patterns. Although the short isoforms are broadly expressed, their physiological functions remain poorly characterized. In this study, we show that short EEF1D isoforms containing exon 5 interact with the ER-resident scaffold protein KTN1 and RRBP1, thereby anchoring the EEF1B complex to the ER. Mass spectrometry analyses of FLAG-tagged EEF1D identified these interactions, and deletion of exon 5 disrupted ER anchoring, resulting in diffuse cytoplasmic localization of the EEF1B complex. In exon 5 KO mice, this altered localization was accompanied by a reduction in EEF1B subunit abundance in multiple tissues, including the liver, although global protein synthesis rates remained unchanged. Together, these findings uncover an ER-anchoring mechanism controlled by alternative splicing that shapes the spatial organization and abundance of the elongation machinery in vivo. |
| 巻・号 | 9(8) |
| 公開日 | 2026-8-1 |
| DOI | 10.26508/lsa.202503513 |
| PII | 9/8/e202503513 |
| PMID | 42230146 |
| PMC | PMC13231004 |
| MeSH | Alternative Splicing* / genetics Animals Endoplasmic Reticulum* / metabolism Exons / genetics Humans Mice Mice, Knockout Organ Specificity / genetics Peptide Elongation Factor 1* / genetics Peptide Elongation Factor 1* / metabolism Protein Isoforms / genetics Protein Isoforms / metabolism |
| IF | 2.622 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 5.8 |
| リソース情報 | |
| ヒト・動物細胞 | Hepa 1-6(RCB1638) |