| RRC ID |
89263
|
| Author |
Fuwa TJ, Hori K, Sasamura T, Higgs J, Baron M, Matsuno K.
|
| Title |
The first deltex null mutant indicates tissue-specific deltex-dependent Notch signaling in Drosophila.
|
| Journal |
Mol Genet Genomics
|
| Abstract |
Notch (N) is a single-pass transmembrane receptor. The N signaling pathway is an evolutionarily conserved mechanism that controls various cell-specification processes. Drosophila Deltex (Dx), a RING-domain E3 ubiquitin ligase, binds to the N intracellular domain, promotes N's endocytic trafficking to late endosomes, and was proposed to activate Suppressor of Hairless [Su(H)]-independent N signaling. However, it has been difficult to evaluate the importance of dx, because no null mutant of a dx family gene has been available in any organism. Here, we report the first null mutant allele of Drosophila dx. We found that dx was involved only in the subsets of N signaling, but was not essential for it in any developmental context. A strong genetic interaction between dx and Su(H) suggested that dx might function in Su(H)-dependent N signaling. Our epistatic analyses suggested that dx functions downstream of the ligands and upstream of activated Su(H). We also uncovered a novel dx activity that suppressed N signaling downstream of N.
|
| Volume |
275(3)
|
| Pages |
251-63
|
| Published |
2006-3-1
|
| DOI |
10.1007/s00438-005-0087-3
|
| PMID |
16395579
|
| MeSH |
Animals
Base Sequence
DNA Primers
Drosophila
Drosophila Proteins / genetics*
Drosophila Proteins / metabolism*
Endocytosis
Membrane Proteins / genetics*
Mosaicism
Mutation*
Receptors, Notch / metabolism*
Signal Transduction*
|
| Resource |
| Drosophila |
DGRC#119830 |
