| RRC ID |
89445
|
| 著者 |
Kurtović K, Vosolsobě S, Nedvěd D, Müller K, Dobrev PI, Schmidt V, Piszczek P, Kuhn A, Smoljan A, Fisher TJ, Weijers D, Friml J, Bowman JL, Petrášek J.
|
| タイトル |
The role of indole-3-acetic acid and characterization of PIN transporters in complex streptophyte alga Chara braunii.
|
| ジャーナル |
New Phytol
|
| Abstract |
Auxin, indole-3-acetic acid (IAA), is a key phytohormone with diverse morphogenic roles in land plants, but its function and transport mechanisms in algae remain poorly understood. We therefore aimed to explore the role of IAA in a complex, streptophyte algae Chara braunii. Here, we described novel responses of C. braunii to IAA and characterized two homologs of PIN auxin efflux carriers: CbPINa and CbPINc. We determined their localization in C. braunii using epitope-specific antibodies and tested their function in heterologous land plant models. Further, using phosphoproteomic analysis, we identified IAA-induced phosphorylation events. The thallus regeneration assay showed that IAA promotes thallus elongation and side branch development. Immunolocalization of CbPINa and CbPINc confirmed their presence on the plasma membrane of vegetative and generative cells of C. braunii. However, functional assays in tobacco BY-2 cells demonstrated that CbPINa affects auxin transport, whereas CbPINc does not. The IAA is effective in the acceleration of cytoplasmic streaming and the phosphorylation of evolutionary conserved targets such as homolog of RAF-like kinase. These findings suggest that, although canonical PIN-mediated auxin transport mechanisms might not be fully conserved in Chara, IAA is involved in morphogenesis and fast signaling processes.
|
| 巻・号 |
246(3)
|
| ページ |
1066-1083
|
| 公開日 |
2025-5-1
|
| DOI |
10.1111/nph.70019
|
| PMID |
40047465
|
| PMC |
PMC11982790
|
| MeSH |
Biological Transport / drug effects
Cell Membrane / drug effects
Cell Membrane / metabolism
Chara* / drug effects
Chara* / metabolism
Indoleacetic Acids* / metabolism
Indoleacetic Acids* / pharmacology
Membrane Transport Proteins* / metabolism
Nicotiana / metabolism
Phosphorylation / drug effects
Plant Proteins* / metabolism
|
| リソース情報 |
| 藻類 |
NIES-1604 |
