論文 - 詳細
| RRC ID | 89473 |
|---|---|
| 著者 | Matsuda K, Wang CH, Kakinuma H, Toyoda A, Shiraki T, Kawakami K, Kubo F. |
| タイトル | Molecular and functional dissection using CaMPARI-seq reveals the neuronal organization for dissociating optic flow-dependent behaviors. |
| ジャーナル | Nat Commun |
| Abstract |
Optic flow processing is critical for the visual control of body and eye movements in many animals. Rotational and translational binocular optic flow patterns need to be clearly distinguished to induce different behavior outputs. However, the specific neuron types and their connectivity involved in this computation remain unclear. Here, we developed a method to link the functional labeling using a photoconvertible calcium indicator called CaMPARI2 and single-cell RNA sequencing (CaMPARI-seq) to investigate the transcriptional profile of the pretectum, a center for processing optic flow in larval zebrafish. Using this technique, we identified a pretectal cluster expressing tcf7l2, which can be further classified into molecularly distinct subclusters. In vivo calcium imaging and cell ablation revealed that nkx1.2lb-positive pretectal neurons are commissural inhibitory neurons required for the optomotor response but not for the optokinetic response. Our genetic and functional dissection using CaMPARI-seq uncovered the neuronal organization essential for dissociating different optic flow-dependent behaviors. |
| 巻・号 | 17(1) |
| 公開日 | 2026-4-17 |
| DOI | 10.1038/s41467-026-71371-6 |
| PII | 10.1038/s41467-026-71371-6 |
| PMID | 41997902 |
| PMC | PMC13090360 |
| MeSH | Animals Animals, Genetically Modified Calcium / metabolism Larva / physiology Neurons* / metabolism Neurons* / physiology Optic Flow* / physiology Single-Cell Analysis / methods Single-Cell Gene Expression Analysis Zebrafish* / genetics Zebrafish* / physiology Zebrafish Proteins / genetics Zebrafish Proteins / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 31 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 15.9 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(UAS:GFP) |