論文 - 詳細
| RRC ID | 89533 |
|---|---|
| 著者 | Wintz K, Schumacher F, Pfeiffer C, Schäfer M, Gering I, Schemmert S, Willuweit A, Kutzsche J. |
| タイトル | In-depth characterization of the CaV2.2-knockout mouse line. |
| ジャーナル | Sci Rep |
| Abstract |
The voltage-gated calcium channel CaV2.2 is predominantly located in neuronal synapses, where it plays a role in pain signaling and neurotransmitter release. Furthermore, CaV2.2 is important during early central nervous system development, as it is subsequently replaced by other calcium channels. A number of mouse lines with disrupted CaV2.2 channels have been developed through the utilization of a gene-targeting vector. The CaV2.2-deficient mice primarily exhibited reduced anxiety-linked behavior and response to pain stimuli. In a previous study, we observed hyperactivity and seizures in CaV2.2-knockout mice. Given the absence of prior descriptions of these characteristics, an in-depth characterization was conducted. This characterization included longitudinal behavioral studies, as well as histological, proteomic, and metabolomic analyses. Irrespective of the frequency with which testing was conducted, a variety of behaviors were observed (e.g., hyperactivity and increased exploratory behavior) in comparison with wild-type mice. The frequency and severity of the performed tests demonstrated a positive correlation with the magnitude of the difference in molecular markers between CaV2.2-knockout and wild-type mice. Seizures were observed but were not common. These results suggest that the CaV2.2-knockout mouse lines are susceptible to repeated stress stimuli during experimental studies. The mice are characterized by hyperactivity, explorative behavior, and sometimes experience seizures. |
| 巻・号 | 16(1) |
| 公開日 | 2026-7-9 |
| DOI | 10.1038/s41598-026-60827-w |
| PII | 10.1038/s41598-026-60827-w |
| PMID | 42426132 |
| PMC | PMC13351052 |
| MeSH | Animals Behavior, Animal Calcium Channels, N-Type* / deficiency Calcium Channels, N-Type* / genetics Calcium Channels, N-Type* / metabolism Caveolin 2 Male Mice Mice, Knockout Seizures / genetics Seizures / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Bluesky |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.5 |
| リソース情報 | |
| 実験動物マウス | RBRC04884 |