| RRC ID |
89533
|
| 著者 |
Wintz K, Schumacher F, Pfeiffer C, Schäfer M, Gering I, Schemmert S, Willuweit A, Kutzsche J.
|
| タイトル |
In-depth characterization of the CaV2.2-knockout mouse line.
|
| ジャーナル |
Sci Rep
|
| Abstract |
The voltage-gated calcium channel CaV2.2 is predominantly located in neuronal synapses, where it plays a role in pain signaling and neurotransmitter release. Furthermore, CaV2.2 is important during early central nervous system development, as it is subsequently replaced by other calcium channels. A number of mouse lines with disrupted CaV2.2 channels have been developed through the utilization of a gene-targeting vector. The CaV2.2-deficient mice primarily exhibited reduced anxiety-linked behavior and response to pain stimuli. In a previous study, we observed hyperactivity and seizures in CaV2.2-knockout mice. Given the absence of prior descriptions of these characteristics, an in-depth characterization was conducted. This characterization included longitudinal behavioral studies, as well as histological, proteomic, and metabolomic analyses. Irrespective of the frequency with which testing was conducted, a variety of behaviors were observed (e.g., hyperactivity and increased exploratory behavior) in comparison with wild-type mice. The frequency and severity of the performed tests demonstrated a positive correlation with the magnitude of the difference in molecular markers between CaV2.2-knockout and wild-type mice. Seizures were observed but were not common. These results suggest that the CaV2.2-knockout mouse lines are susceptible to repeated stress stimuli during experimental studies. The mice are characterized by hyperactivity, explorative behavior, and sometimes experience seizures.
|
| 巻・号 |
16(1)
|
| 公開日 |
2026-7-9
|
| DOI |
10.1038/s41598-026-60827-w
|
| PII |
10.1038/s41598-026-60827-w
|
| PMID |
42426132
|
| PMC |
PMC13351052
|
| MeSH |
Animals
Behavior, Animal
Calcium Channels, N-Type* / deficiency
Calcium Channels, N-Type* / genetics
Calcium Channels, N-Type* / metabolism
Caveolin 2
Male
Mice
Mice, Knockout
Seizures / genetics
Seizures / metabolism
|
| リソース情報 |
| 実験動物マウス |
RBRC04884 |
