Reference - Detail
| RRC ID | 89782 |
|---|---|
| Author | Hirata M, Fujino M, Ichihara K, Tang R, Narita M, Iwasaki W, Shiraishi C, Shiraishi T, Hatano A, Suzuki T, Abe T, Takami T, Mishiro-Sato E, Toyoda A, Matsumoto M, Tanaka Y, Ito T, Inada T, Nakayama KI, Yokoyama T, Matsumoto A. |
| Title | RPL41 stabilizes ribosome dynamics and supports long-protein homeostasis in mammals. |
| Journal | Nucleic Acids Res |
| Abstract |
Ribosomal protein L41 (RPL41 or eL41) is the smallest ribosomal protein and forms the eukaryote-specific bridge, eB14, near the decoding center; however, its role in mammalian translation remains unclear. In this study, we established RPL41-deficient models of human HEK293T cells and mice to define its function. Cryo-electron microscopy revealed that RPL41 constrains intersubunit conformational dynamics without inducing major local static rearrangements. Loss of RPL41 altered A-site dynamics, slowed elongation, modestly increased amino acid misincorporation, and modestly enhanced readthrough of collision-inducing reporter sequences. Quantitative proteomic analysis suggested that these translational defects compromise long-protein homeostasis, as evidenced by increased insolubility and reduced abundance of long proteins. In vivo, Rpl41-/- mice were viable but exhibited growth retardation and decreased abundance of long proteins in tissues. Our findings reveal a conserved role for RPL41 in maintaining ribosome dynamics and translational fidelity, indicating that RPL41 supports ribosome function and long-protein homeostasis in mammals. |
| Volume | 54(15) |
| Published | 2026-8-10 |
| DOI | 10.1093/nar/gkag797 |
| PII | 8760021 |
| PMID | 42613035 |
| PMC | PMC13485352 |
| MeSH | Animals HEK293 Cells Homeostasis Humans Mice Mice, Knockout Protein Biosynthesis* Proteostasis* Ribosomal Proteins* / chemistry Ribosomal Proteins* / genetics Ribosomal Proteins* / metabolism Ribosomal Proteins* / physiology Ribosomes* / chemistry Ribosomes* / metabolism Ribosomes* / ultrastructure |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 9 |
| Altmetric score changes over past 6months | 11.3 |
| Resource | |
| Human and Animal Cells | 293T(RCB2202) |