Reference - Detail
| RRC ID | 89863 |
|---|---|
| Author | Kirimura N, Ueno S, Yamazaki R, Tsukamoto Y. |
| Title | Anti-gicerin Antibody Suppresses Early Pulmonary Metastatic Colonization of B16F10 Melanoma Cells. |
| Journal | In Vivo |
| Abstract |
BACKGROUND/AIM:Adhesion of circulating melanoma cells to the pulmonary vascular bed is a critical early step in hematogenous metastasis. Gicerin is an immunoglobulin superfamily cell adhesion molecule that can mediate homophilic cell-cell adhesion. We examined whether gicerin is expressed in B16F10 melanoma cells and pulmonary tissue and whether systemic anti-gicerin antibody suppresses experimental pulmonary colonization. MATERIALS AND METHODS:Gicerin expression was examined by immunofluorescence staining in cultured B16F10 cells, subcutaneous B16F10 tumors, pulmonary metastatic foci, and normal lung tissue, with matched rabbit pre-immune IgG controls processed in parallel. Histopathological features of subcutaneous and pulmonary lesions were evaluated by hematoxylin and eosin staining. Nude mice received a tail-vein injection of B16F10 cells followed immediately by intravenous administration of matched rabbit pre-immune IgG or rabbit polyclonal anti-gicerin IgG. Cell proliferation, adhesion to human umbilical vein endothelial cells (HUVECs), and wound-healing migration were assessed in vitro. RESULTS:Gicerin immunoreactivity was detected in cultured B16F10 cells, subcutaneous tumors, pulmonary metastatic foci, and normal pulmonary structures, whereas matched rabbit pre-immune IgG controls showed no specific fluorescence, supporting staining specificity under the assay conditions. Tail-vein injection produced early pulmonary metastatic foci, and anti-gicerin IgG at 10 μg/mouse significantly reduced metastatic colonization compared with pre-immune IgG, whereas the 1 μg/mouse dose did not. Anti-gicerin IgG significantly inhibited B16F10 adhesion to HUVEC monolayers and significantly reduced cell migration without significantly affecting cell proliferation. CONCLUSION:Gicerin contributes to early pulmonary colonization of B16F10 melanoma cells by supporting tumor-endothelial adhesion and tumor-cell migration. Gicerin blockade may represent a potential strategy for preventing hematogenous dissemination of melanoma. |
| Volume | 40(5) |
| Pages | 2635-2651 |
| Published | 2026-1-1 |
| DOI | 10.21873/invivo.14418 |
| PII | 40/5/2635 |
| PMID | 42665396 |
| PMC | PMC13531101 |
| MeSH | Animals Antibodies* / pharmacology Cell Adhesion / drug effects Cell Line, Tumor Cell Movement / drug effects Cell Proliferation / drug effects Female Human Umbilical Vein Endothelial Cells Humans Lung Neoplasms* / drug therapy Lung Neoplasms* / immunology Lung Neoplasms* / metabolism Lung Neoplasms* / pathology Lung Neoplasms* / secondary Melanoma, Experimental* / drug therapy Melanoma, Experimental* / immunology Melanoma, Experimental* / metabolism Melanoma, Experimental* / pathology Mice Rabbits |
| Resource | |
| Human and Animal Cells | B16F10(RCB2630) |