| RRC ID |
951
|
| 著者 |
Kucsera J, Yarita K, Takeo K, Yoshida S, Gácser A, Hamari Z, Avasi Z, Kevei F.
|
| タイトル |
Intra-strain variability of Cryptococcus neoformans can be detected on phloxin B medium.
|
| ジャーナル |
J Basic Microbiol
|
| Abstract |
A method was devised for easy detection of intra-strain variability of the human pathogenic yeast Cryptococcus neoformans. Cultivation of strains on a medium containing Phloxin B resulted in different coloured colonies. Generally, colonies were either pink or red; however there were also several colony-colour segregant in which both colours could be observed. A number of these segregants were isolated and analysed. Virulence factors such as the cell and capsule sizes were measured; further temperature sensitivity, growth rates, mating-types and melanin production were also studied. Segregants were examined by random amplified polymorphic DNA (RAPD) fingerprinting and electrophoretic karyotyping by pulsed-field gel electrophoresis (CHEF). They showed both phenotypic and genotypic differences. The main differences appeared in phenotypic characters and RAPD patterns; while the chromosomal patterns remained unchanged. Reversion frequency analysis revealed that the reason for this segregation could be due to phenotypic switching. The physiological reason for the colour changes was also investigated and was attributed to the differential ability of the cells to accumulate Phloxin B either into their capsules or into their cells. The method described here is potentially applicable for the detection of strain heterogeneity in both basic and clinical microbiology laboratories.
|
| 巻・号 |
42(2)
|
| ページ |
111-9
|
| 公開日 |
2002-1-1
|
| DOI |
10.1002/1521-4028(200205)42:2<111::AID-JOBM111>3.0.CO;2-3
|
| PMID |
11981875
|
| MeSH |
Cryptococcus neoformans / classification
Cryptococcus neoformans / genetics
Cryptococcus neoformans / growth & development*
Cryptococcus neoformans / metabolism
Culture Media / metabolism
DNA Fingerprinting
Electrophoresis, Gel, Pulsed-Field
Eosine I Bluish / metabolism*
Fluorescent Dyes / metabolism
Genetic Variation / genetics
Mycological Typing Techniques / methods
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
Species Specificity
|
| IF |
1.909
|
| 引用数 |
3
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
IFM 40038
IFM 47841
IFM 47842
IFM 47843
IFM 47844
IFM 47845
IFM 47847
IFM 47849
IFM 47853
IFM 47855
IFM 47856
IFM 47857
IFM 47865
IFM 47866
IFM 47867
IFM 47868
IFM 47871
IFM 47872
IFM 47873
IFM 47876
IFM 5844
IFM 5845 |
