| RRC ID |
961
|
| Author |
Mimuro H, Suzuki T, Tanaka J, Asahi M, Haas R, Sasakawa C.
|
| Title |
Grb2 is a key mediator of helicobacter pylori CagA protein activities.
|
| Journal |
Mol Cell
|
| Abstract |
CagA delivered from Helicobacter pylori into gastric epithelial cells undergoes tyrosine phosphorylation and induces host cell morphological changes. Here we show that CagA can interact with Grb2 both in vitro and in vivo, which results in the activation of the Ras/MEK/ERK pathway and leads to cell scattering as well as proliferation. Importantly, this ability of CagA is independent from the tyrosine phosphorylation, which occurs within the five repeated EPIYA sequences (PY region) of CagA. However, the PY region appears to be indispensable for the Grb2 binding and induction of the cellular responses. Thus, intracellular CagA via its binding to Grb2 may act as a transducer for stimulating growth factor-like downstream signals which lead to cell morphological changes and proliferation, the causes of H. pylori-induced gastric hyperplasia.
|
| Volume |
10(4)
|
| Pages |
745-55
|
| Published |
2002-10-1
|
| DOI |
10.1016/s1097-2765(02)00681-0
|
| PII |
S1097-2765(02)00681-0
|
| PMID |
12419219
|
| MeSH |
Adaptor Proteins, Signal Transducing*
Animals
Antigens, Bacterial*
Bacterial Proteins / chemistry
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
COS Cells
Cell Division
Cell Line
Cell Movement
Cell Size
GRB2 Adaptor Protein
Helicobacter pylori / genetics
Helicobacter pylori / metabolism*
Humans
Mutation
Phosphorylation
Phosphotyrosine / metabolism
Protein Binding
Proteins / genetics
Proteins / metabolism*
Signal Transduction
|
| IF |
15.584
|
| Times Cited |
267
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Pathogenic microorganisms |
IID 3022(ATCC 43579)?
IID 3023(NCTC 11637)?
IID 6171(26695)? |
