論文 - 詳細
| RRC ID | 964 |
|---|---|
| 著者 | Suzuki T, Mimuro H, Suetsugu S, Miki H, Takenawa T, Sasakawa C. |
| タイトル | Neural Wiskott-Aldrich syndrome protein (N-WASP) is the specific ligand for Shigella VirG among the WASP family and determines the host cell type allowing actin-based spreading. |
| ジャーナル | Cell Microbiol |
| Abstract |
Shigella, the causative agent of bacillary dysentery, is capable of directing its movement within host cells by forming an actin comet tail. The VirG (IcsA) pro-tein expressed at one pole of the bacterium recruits neural Wiskott-Aldrich syndrome protein (N-WASP), a member of the WASP family, which in turn stimulates actin-related protein (Arp) 2/3 complex-mediated actin polymerization. As all the WASP family proteins induce actin polymerization by recruiting Arp2/3 complex, we investigated their involvement in Shigella motility. Here, we show that VirG binds to N-WASP but not to the other WASP family proteins. Using a series of chimeras obtained by swapping N-WASP and WASP domains, we demonstrated that the specificity of VirG to interact with N-WASP lies in the N-terminal region containing the pleckstrin homology (PH) domain and calmodulin-binding IQ motif of N-WASP. A conformational change in N-WASP was important for the VirG-N-WASP interaction, as elimination of the C-terminal acidic region, which is responsible for the intramolecular interaction with the central basic region of N-WASP, affected the specific binding to VirG. We observed that, in haematopoietic cells such as macrophages, polymorphonuclear leucocytes (PMNs) and platelets, WASP was predominantly expressed, whereas the expression of N-WASP was greatly suppressed. Indeed, unlike Listeria, Shigella was unable to move in macrophages at all, although the movement was restored as N-WASP was expressed ectopically. Thus, our findings demonstrate that N-WASP is a specific ligand of VirG, which determines the host cell type allowing actin-based spreading of Shigella. |
| 巻・号 | 4(4) |
| ページ | 223-33 |
| 公開日 | 2002-4-1 |
| DOI | 10.1046/j.1462-5822.2002.00185.x |
| PMID | 11952639 |
| MeSH | Actins / chemistry Actins / metabolism* Bacterial Proteins Biological Transport Cells, Cultured DNA-Binding Proteins / metabolism* Humans Ligands Macrophages / metabolism Macrophages / microbiology Nerve Tissue Proteins / metabolism* Shigella / metabolism* Signal Transduction Transcription Factors / metabolism* Wiskott-Aldrich Syndrome Protein, Neuronal |
| IF | 3.43 |
| 引用数 | 57 |
| WOS 分野 | MICROBIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 病原微生物 | S. flexneri 2a? L. monocytogenes(IID 6001)? |