論文 - 詳細
| RRC ID | 969 |
|---|---|
| 著者 | Ootsubo M, Shimizu T, Tanaka R, Sawabe T, Tajima K, Yoshimizu M, Ezura Y, Ezaki T, Oyaizu H. |
| タイトル | Oligonucleotide probe for detecting Enterobacteriaceae by in situ hybridization. |
| ジャーナル | J Appl Microbiol |
| Abstract |
AIMS:To develop oligonucleotide probes for visualizing bacteria belonging to Enterobacteriaceae. METHODS AND RESULTS:24-mer oligonucleotide probe (probe D) was designed by comparison of 16S rDNA sequences of 35 species of Enterobacteriaceae, eight species of Vibrionaceae and six species of Pasteurellaceae. The sequence of the probe corresponding to the complementary sequence of a position 1251-1274 of Escherichia coli 16S rRNA was found to be a highly conserved region of 16S rDNA sequence in Enterobacteriaceae different from that of Vibrionaceae and Pasteurellaceae. The fluorescent dye-labelled probe was tested for the specificity by in situ hybridization and epifluorescence microscopy. Seventy-six out of 78 strains belonging to Enterobacteriaceae were visualized in an optimal hybridization condition. No bacterial strains belonging to Vibrionaceae (31 strains) and Gram-positive bacteria (three strains) were visualized. CONCLUSIONS:In situ hybridization using probe D allows the detection of bacterial cells belonging to Enterobacteriaceae without false positive reaction. SIGNIFICANCE AND IMPACT OF THE STUDY:In situ hybridization techniques using the probe D are potential tools for detecting Enterobacteriaceae in food and water samples. |
| 巻・号 | 93(1) |
| ページ | 60-8 |
| 公開日 | 2002-1-1 |
| DOI | 10.1046/j.1365-2672.2002.01668.x |
| PII | 1668 |
| PMID | 12067375 |
| MeSH | DNA, Bacterial / analysis DNA, Ribosomal / analysis Enterobacteriaceae / genetics* Enterobacteriaceae / isolation & purification* Food Microbiology In Situ Hybridization, Fluorescence / methods* Oligonucleotide Probes / genetics RNA, Ribosomal, 16S / analysis Sensitivity and Specificity Water Microbiology Water Supply / standards* |
| IF | 3.066 |
| 引用数 | 41 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 病原微生物 | JCM 1090 JCM 1684 JCM 7582 JCM 1659 JCM 1657 JCM 1237 JCM 6051 JCM 3943 JCM 1234 GTC 01061 JCM 1473 JCM 5911 JCM 1666 JCM 6096 JCM 1663 JCM 1687 JCM 1681 JCM 1682 JCM 1667 JCM 5895 JCM 8882 JCM 1683 GTC 01735? GTC 01733? JCM 5910 JCM 1243 JCM 1244 GTC 786? GTC 00781 JCM 3902 JCM 1685 JCM 1679 JCM 1656 JCM 1650 JCM 1664 JCM 1672 JCM 8885 JCM 1669 JCM 1670 JCM 1668 JCM 3948 JCM 1673 JCM 1675 JCM 3953 JCM 1651 GTC 01734? GTC 01731? JCM 1245 GTC 00779 GTC 00780 JCM 5892 JCM 7579 JCM 7376 JCM 2429 JCM 2403 JCM 1060 JCM 8238 JCM 1043 JCM 2385 JCM 7875 JCM 7873 JCM 7874 JCM 7373 JCM 2139 JCM 7375 JCM 8968 JCM 10084 JCM 1283 JCM 3725 |