Author Kuwamura M, Kanehara T, Tokuda S, Kumagai D, Yamate J, Kotani T, Nakane Y, Kuramoto T, Serikawa T.
Title Immunohistochemical and morphometrical studies on myelin breakdown in the demyelination (dmy) mutant rat.
Journal Brain Res
Abstract The demyelination (dmy) rat is a unique mutant exhibiting severe myelin breakdown in the central nervous system (CNS). In this study, we conducted immunohistochemical and morphometrical investigations in the dmy rat. From around 6 weeks of age, the affected rats developed ataxia especially in the hindlimbs. Afterwards, ataxia worsened rapidly, resulting in complete paralysis of the hindlimbs and recumbency. Histopathology at 7 to 10 weeks of age revealed myelin destruction throughout the white matter of the CNS in the dmy rats. The most severely affected lesions were distributed in the corpus callosum, capsula interna, striatum, subcortical white matter, cerebellar peduncle, and ventral and lateral parts of the spinal cord. Immunohistochemistry demonstrated prominent astrogliosis and many ED-1 positive macrophages in the myelin-destructed areas. Until the 4th week, no significant differences in myelin thickness and fiber diameter were found between dmy and control rats. However, from 5 weeks of age, myelin thickness of residual myelinated fibers in dmy rats became significantly less than that in controls. These data indicated that the dmy phenotype shows a prolonged period of myelin destruction, suggesting that dmy mutation affects the adequate maintenance of myelin.
Volume 1022(1-2)
Pages 110-6
Published 2004-10-1
DOI 10.1016/j.brainres.2004.07.007
PII S0006-8993(04)01083-2
PMID 15353220
MeSH Age Factors Animals Axons / pathology Ciliary Neurotrophic Factor Demyelinating Diseases / metabolism Demyelinating Diseases / pathology* Disease Models, Animal Glial Fibrillary Acidic Protein / metabolism Histological Techniques / methods Immunohistochemistry / methods Microscopy, Electron, Transmission / methods Myelin Basic Protein / metabolism Myelin Sheath / genetics Myelin Sheath / pathology* Myelin Sheath / ultrastructure Rats Rats, Mutant Strains Rats, Sprague-Dawley Spinal Cord / metabolism Spinal Cord / pathology
IF 2.733
Times Cited 8
Rats WTC/Kyo(strainID=18)