RRC ID |
49427
|
著者 |
Sun P, Gao JL, Mao XJ, Zhao XH, Sun JG, Lu M.
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タイトル |
Lentibacillus populi sp. nov., a moderately halophilic, endophytic bacterium isolated from a poplar tree, and emended description of the genus Lentibacillus.
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ジャーナル |
Int J Syst Evol Microbiol
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Abstract |
A Gram-staining-positive, moderately halophilic, aerobic, endospore-forming, rod-shaped bacterial strain, designated WD4L-1T was isolated from surface-sterilized stem tissue of a poplar tree planted in the Wudalianchi National Geopark of Heilongjiang province, PR China. This novel isolate grew in the presence of 0-15 % (w/v) NaCl, at pH 6.0-9.0 and 15-50 °C; optimum growth was observed with 7-8 % (w/v) NaCl, at pH 7.0 and 30 °C. The 16S rRNA gene sequence analysis indicated that the strain WD4L-1T belonged to the genus Lentibacillus, and was most closely related to Lentibacillus garicola SL-MJ1T with a sequence similarity of 96.1 %. The DNA G+C content of strain WD4L-1T was determined to be 36.9 mol%. The respiratory quinone was identified as menaquinone-7 (MK-7) and the major lipids were diphosphatidylglycerol and phosphatidylglycerol and one unidentified phospholipid. The major fatty acids of strain WD4L-1T were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0. The results of the physiological and biochemical tests and the minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain WD4L-1T from the closely related Lentibacillus garicola JCM 30131T. Thus, strain WD4L-1T represents a novel species of the genus Lentibacillus, for which the name Lentibacillus populi sp. nov. is proposed. The type strain is WD4L-1T (=CGMCC 1.15454T=DSM 101738T). An emended description of the genus Lentibacillus is also provided.
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巻・号 |
66(12)
|
ページ |
5281-5287
|
公開日 |
2016-12-1
|
DOI |
10.1099/ijsem.0.001508
|
PMID |
27652598
|
MeSH |
Bacillaceae / classification*
Bacillaceae / genetics
Bacillaceae / isolation & purification
Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial / genetics
Fatty Acids / chemistry
Phospholipids / chemistry
Phylogeny*
Populus / microbiology*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Trees / microbiology
Vitamin K 2 / analogs & derivatives
Vitamin K 2 / chemistry
|
IF |
2.415
|
引用数 |
6
|
リソース情報 |
一般微生物 |
JCM 30131
JCM 15395 |