RRC ID |
29835
|
著者 |
Kogoma T, Subia NL, von Meyenburg K.
|
タイトル |
Function of ribonuclease H in initiation of DNA replication in Escherichia coli K-12.
|
ジャーナル |
Mol Gen Genet
|
Abstract |
Escherichia coli rnh mutants lacking ribonuclease H (RNase H) activity can tolerate deletion of the origin of DNA Replication (delta oriC) and transposon-insertional inactivation of an initiator gene (dnaA::Tn10). Introduction of the recA200 allele encoding a thermolabile RecA protein into rnh- dnaA::Tn10 and rnh- delta oriC mutants strains rendered DNA synthesis and colony formation of these mutants temperature sensitive. The temperature sensitivity and the broth sensitivity (Srm-) of the rnh- dnaA::Tn10 recA200 strain was suppressed by the presence of plasmids (pBR322 derivatives) carrying dnaA+ only when the intact oriC site was present on the chromosome. Lack of RNase H activity neither promoted replication of minichromosomes (pOC24 and p lambda asn20) in the absence of required DnaA+ protein nor inhibited dnaA+-dependent minichromosome replication. These results led to the conclusion that RNase H is not directly involved in the events leading to initiation of DNA replication at oriC. Rather, it functions as a specificity factor by eliminating certain forms of RNA-DNA hybrids which could otherwise be used to prime DNA replication at sites other than oriC.
|
巻・号 |
200(1)
|
ページ |
103-9
|
公開日 |
1985-1-1
|
DOI |
10.1007/BF00383320
|
PMID |
2993805
|
MeSH |
Chromosome Deletion
DNA Replication*
DNA Restriction Enzymes
Endoribonucleases / metabolism*
Escherichia coli / enzymology*
Escherichia coli / genetics
Genotype
Kinetics
Mutation
Plasmids
Ribonuclease H
Species Specificity
Thermodynamics
|
引用数 |
40
|
WOS 分野
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
原核生物(大腸菌) |
ME9199
ME9202
ME9204
ME9195
ME9235
ME9196
ME9198 |