RRC ID |
49409
|
著者 |
Zhang B, Tang S, Chen X, Zhang L, Zhang G, Zhang W, Liu G, Chen T, Li S, Dyson P.
|
タイトル |
Streptomyces lacrimifluminis sp. nov., a novel actinobacterium that produces antibacterial compounds, isolated from soil.
|
ジャーナル |
Int J Syst Evol Microbiol
|
Abstract |
A novel actinobacterial strain, designated Z1027T, was isolated from a soil sample collected near the Tuotuo River, Qinghai-Tibet Plateau (China). The strain exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus. The taxonomic position of strain Z1027T was determined using a polyphasic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Streptomyces and formed a distinct phyletic line in the 16S rRNA gene tree, together with Streptomyces turgidiscabies ATCC 700248T (99.19 % similarity), Streptomyces graminilatus JL-6T (98.84 %) and Streptomyces reticuliscabiei CFBP 4531T (98.36 %). The genomic DNA G+C content of strain Z1027T was 74±1 mol%. The DNA-DNA relatedness values between strain Z1027T and Streptomyces turgidiscabies ATCC 700248T and Streptomyces reticuliscabiei CFBP 4531T were 38.5±0.4 and 26.2±1.2 %, respectively, both of them significantly lower than 70 %. Chemotaxonomic data revealed that strain Z1027T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, ll-diaminopimelic acid as the diagnostic diamino acid and galactose as a whole-cell sugar. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatydilinositol and seven other unknown polar lipids were detected; iso-C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0 were the major fatty acids. On the basis of these genotypic and phenotypic data, it is proposed that isolate Z1027T (=CGMCC 4.7272T=JCM 31054T) should be classified as the type strain of a novel species of the genus Streptomyces,Streptomyces lacrimifluminis sp. nov.
|
巻・号 |
66(12)
|
ページ |
4981-4986
|
公開日 |
2016-12-1
|
DOI |
10.1099/ijsem.0.001456
|
PMID |
27601150
|
MeSH |
Anti-Bacterial Agents / biosynthesis*
Antibiosis
Bacterial Typing Techniques
Base Composition
China
DNA, Bacterial / genetics
Diaminopimelic Acid / chemistry
Fatty Acids / chemistry
Nucleic Acid Hybridization
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil Microbiology*
Streptomyces / classification*
Streptomyces / genetics
Streptomyces / isolation & purification
Vitamin K 2 / analogs & derivatives
Vitamin K 2 / chemistry
|
IF |
2.415
|
引用数 |
5
|
リソース情報 |
一般微生物 |
JCM 31054
JCM 10429 |