RRC ID |
48144
|
著者 |
Wang JL, Ma KD, Wang YW, Wang HM, Li YB, Zhou S, Chen XR, Kong DL, Guo X, He MX, Ruan ZY.
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タイトル |
Lentibacillus amyloliquefaciens sp. nov., a halophilic bacterium isolated from saline sediment sample.
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ジャーナル |
Antonie Van Leeuwenhoek
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Abstract |
A Gram-stain positive, non-motile, non-sporogenous, aerobic, rod-shaped and halophilic bacterium, designated LAM0015(T), was isolated from a saline sediment sample collected from Yantai City in China. The isolate was found to be able to grow at NaCl concentrations of 5-25 % (w/v) (optimum: 7-12 %), 15-45 °C (optimum: 35 °C) and pH 5.0-9.0 (optimum: 7.0). The major fatty acids were determined to be anteiso-C15:0 and anteiso-C17:0. The predominant respiratory quinone was identified as MK-7. The cell wall peptidoglycan was determined to contain meso-diaminopimelic acid. The polar lipids were found to be diphosphatidyglycerol, phosphatidylglycerol, five phospholipids and one glycolipid. The DNA G+C content was 43.1 mol% as determined by the T m method. Analysis of the 16S rRNA gene sequence indicated that the isolate belongs within the genus Lentibacillus and is closely related to Lentibacillus persicus DSM 22530(T), Lentibacillus salicampi JCM 11462(T) and Lentibacillus jeotgali JCM 15795(T) with 97.3, 96.7 and 96.4 % sequence similarity, respectively. The DNA-DNA hybridization value between LAM0015(T) and L. persicus DSM 22530(T) was 51.2 ± 1.4 %. Based on its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM0015(T) is concluded to represent a novel species of the genus Lentibacillus, for which the name Lentibacillus amyloliquefaciens sp. nov. is proposed. The type strain is LAM0015(T) (=ACCC 06401(T) = JCM 19838(T)).
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巻・号 |
109(2)
|
ページ |
171-8
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公開日 |
2016-2-1
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DOI |
10.1007/s10482-015-0618-9
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PII |
10.1007/s10482-015-0618-9
|
PMID |
26545789
|
MeSH |
Bacillaceae / classification
Bacillaceae / genetics
Bacillaceae / isolation & purification*
Bacillaceae / metabolism
Base Composition
China
DNA, Bacterial / genetics
Fatty Acids / chemistry
Fatty Acids / metabolism
Geologic Sediments / analysis
Geologic Sediments / microbiology*
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S / genetics
Sodium Chloride / analysis
Sodium Chloride / metabolism*
|
IF |
2.674
|
引用数 |
8
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 11462
JCM 15795
JCM 19838 |