RRC ID |
28121
|
著者 |
Hooks KB, Turner JE, Graham IA, Runions J, Hooks MA.
|
タイトル |
GFP-tagging of Arabidopsis acyl-activating enzymes raises the issue of peroxisome-chloroplast import competition versus dual localization.
|
ジャーナル |
J Plant Physiol
|
Abstract |
Protein sequence analysis of a subfamily of 18 Arabidopsis acyl-activating enzymes (AAE) for organelle targeting signals revealed that eight of them possessed putative peroxisomal targeting signals (PTS1), five of which belonged to Clade VI of the AAE superfamily. Peroxisomal localization was confirmed by confocal microscopy of green fluorescent protein (GFP)-AAE fusion proteins co-localizing with peroxisomal RFP. The sequence analysis also revealed that all enzymes of Clade VI possess N-terminal regions indicative of chloroplast transit peptides (cTP). Among the five Clade VI peroxisomal enzymes tested, masking the PTS1 signal with GFP redirected three to plastids. In addition, three other peroxisomal AAEs appeared to be redirected to plastids in AAE-GFP fusion constructs. Due to the lack of evidence supporting plastid localization, we propose that competition dictates the exclusive localization to peroxisomes. AAE2 of Clade VI was the only enzyme with a putative mitochondrial targeting sequence, and it appeared to be targeted to mitochondria. The remainder of the AAEs appeared to be localized to plastids or cytosol. The AAE9-GFP fusion protein appeared to be located within discreet structures within plastids that may be plastoglobules. AAE15-GFP, but not AAE16-GFP appeared to be located in the chloroplast envelope. The number of examples is increasing whereby proteins located within other compartments contribute to plastid function. We provide an example of this through the light-sensitive phenotype of mutants of AAE2.
|
巻・号 |
169(16)
|
ページ |
1631-8
|
公開日 |
2012-11-1
|
DOI |
10.1016/j.jplph.2012.05.026
|
PII |
S0176-1617(12)00265-9
|
PMID |
22920973
|
MeSH |
Amino Acid Sequence
Arabidopsis / enzymology*
Arabidopsis / genetics
Arabidopsis / radiation effects
Arabidopsis / ultrastructure
Arabidopsis Proteins / metabolism
Chloroplasts / enzymology
Chloroplasts / metabolism*
Coenzyme A Ligases / metabolism*
Cytosol / enzymology
Cytosol / metabolism
Green Fluorescent Proteins
Light
Luminescent Agents
Microscopy, Confocal
Mitochondria / enzymology
Mitochondria / metabolism*
Molecular Sequence Data
Mutation
Peroxisomes / enzymology
Peroxisomes / metabolism*
Phenotype
Phylogeny
Plants, Genetically Modified
Protein Transport
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Seedlings / enzymology
Seedlings / genetics
Seedlings / radiation effects
Seedlings / ultrastructure
Sequence Alignment
|
IF |
3.013
|
引用数 |
12
|
WOS 分野
|
PLANT SCIENCES
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pda01713
pda04716
pda06578
pda12997
pda04364
pda10158
pda11718
pda08298
pda06811
pda04511
pda09591
pda12714
pda02888
pdy01839
pdy07652 |