RRC ID |
49561
|
著者 |
Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M.
|
タイトル |
CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.
|
ジャーナル |
Nat Methods
|
Abstract |
Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0.
|
巻・号 |
14(10)
|
ページ |
963-966
|
公開日 |
2017-10-1
|
DOI |
10.1038/nmeth.4430
|
PII |
nmeth.4430
|
PMID |
28892089
|
MeSH |
CRISPR-Cas Systems*
Cell Differentiation
Cells, Cultured
DNA, Complementary
Fibroblasts
Gene Expression Regulation / physiology
Humans
Light
Luminescent Measurements
Neurons / physiology*
Optogenetics / methods*
Transcription, Genetic
|
IF |
30.822
|
引用数 |
48
|
リソース情報 |
遺伝子材料 |
pCAGGS (RDB08938). |
ヒト・動物細胞 |
HFSKF-II(RCB0698)
201B7(HPS0063)
454E2(HPS0077) |